离子注入改良维生素C二步发酵混合菌研究(Ⅱ)2-酮基-L-古龙酸高产菌系IPPM-1028发酵条件研究  

Improvement of mingle strains in the fermentation of 2 keto L gulonic acid precursor of Vc by ion implantation (Ⅱ) fermentation conditions of high yield strains IPPM 1028 of 2 keto L gulonic acid

作　　者：许安[1] 姚建铭[1] 余增亮[1] 

机构地区：[1]中国科学院等离子体物理研究所

出　　处：《工业微生物》1999年第2期16-19,共4页Industrial Microbiology

基　　金：国家"九五"攻关项目

摘　　要：考察了不同碳氮源、碳氮比、金属离子和有机溶剂等以及环境因子，如起始ｐＨ，通气量和种龄等对高产菌系ＩＰＰＭ－１０２８发酵水平的影响。实验结果表明，适宜的碳氮比对产酸有明显的影响；在培养基中加入适量的金属离子如Ｍｎ２＋、Ｃｅ３＋、Ｃｅ４＋或Ｌａ２＋以及增加通气量均有利于２－ＫＬＧ的产生。二甲苯和乙酸己酯对产酸也有一定的刺激作用。发酵周期一般为６０～６４ｈ，２－ＫＬＧ浓度可达７６ｇ／ｌ左右，转化率约为９５％。Factors affecting the production of 2 keto L glulonic ac id such as carbon sources,nitrogen sources,carbon nitrogen ratio,metal ions,ini tial pH,etc.were investigated.Results showed that:the suitable proportion of car bon source to nitrogen source was important to the production;the addition of me tal ion such as Mn 2+ ,Ce 3+ ,Ce 4+ and La 2+ was good for the yield and the increment of aeration rate was suitable for the conversion.Dimeth yl benzene and ethyl acetate could irritate the yield slightly.The fermentation period in shake flasks was only 60 64 hours.The yield was kept at 76g/L and the conversion rate was about 95%.

关 键 词：山梨糖 2-KLG 发酵条件 维生素C 

分 类 号：TQ924[轻工技术与工程—发酵工程]