南山楂叶黄酮抑制α-葡萄糖苷酶和清除DPPH自由基的作用  

Alpha-glucosidase Inhibiting Effect of DPPH free Radical Scavenging Activity of Flavonoid-rich Extract from Leaves of Crataegus cuneata Sieb. et Zucc

作　　者：董华强[1] 甄畅迪[1] 张毅[2] 李梅[1] 刘富来[1] 黄剑波[1] 

机构地区：[1]佛山科学技术学院食品科学系,广东佛山528231 [2]华南理工大学轻工与食品学院,广东广州510641

出　　处：《食品科学》2010年第19期179-181,共3页Food Science

基　　金：广东省自然科学基金项目(8152800001000023)

摘　　要：目的:研究南山楂叶黄酮提取物(SZ)对α-葡萄糖苷酶活性的抑制作用和抗氧化活性。方法:检测SZ对来源于酵母菌的α-葡萄糖苷酶活性抑制作用,并采用Lineweave-Burk双倒数曲线研究其抑制机理;测定SZ对DPPH自由基的清除率,考察其抗氧化活性。结果:SZ对α-葡萄糖苷酶的抑制率可达到64.3%,属于非竞争性抑制;对DPPH自由基的清除率可达52.8%,IC50值为0.57mg/mL。结论:SZ对α-葡萄糖苷酶有较强的抑制作用和具有一定的抗氧化活性。A flavonoid-rich extract obtained by ethyl acetate extraction from the 80% ethanol extract from the leaves of Crataegus cuneata Sieb. et Zucc was evaluated for its alpha-glucosidase inhibiting effect and DPPH free radical scavenging activity. The extract had an inhibitory effect on alpha-glucosidase derived from yeast,and the maximum inhibitory rate was up to 64.3%,and the inhibition was uncompetitive according to the Lineweaver-Burk plot. The maximum DPPH free radical scavenging rate of the extract was up to 52.8%,and the IC50 was 0.57 mg/mL. In conclusion,the extract had strong ability to inhibit yeast-derived alpha-glucosidase and certain antioxidant activity.

关 键 词：南山楂叶 黄酮 Α-葡萄糖苷酶 抑制作用 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]