响应面法优化离子交换法固定化β-D-呋喃果糖苷酶  

Optimization of Ion Exchange Immobilization of β-Fructofuranosidase Using Response Surface Methodology

作　　者：张媛媛[1] 聂少平[1] 万成[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品科学》2010年第19期236-240,共5页Food Science

基　　金：教育部"长江学者和创新团队发展计划"项目(IRT0540);食品科学与技术国家重点实验室目标导向资助项目(SKLF-MB-200806)

摘　　要：以大孔阴离子树脂D311为载体,对日本曲霉来源的β-D-呋喃果糖苷酶进行离子交换法固定化。研究温度、pH值、时间、游离酶液酶活力对固定化效果的影响,并在此基础上运用响应面法对固定化条件进行优化。结果表明,最佳固定化条件为:室温、pH6.6、固定化时间4h、游离酶液酶活力为900U/mL,在此条件下,固定化β-D-呋喃果糖苷酶生产的低聚果糖产量可达58.16%。D311 type macroporous anion exchange resin was used as a carrier for the immobilization of β-fructofuranosidase derived from Aspergillus japonicus. The effects of immobilization time and temperature,pH and enzyme activity of free β-fructofuranosidase solution on the catalytic ability of immobilized β-fructofuranosidase were investigated by single factor method and based on this,except immobilization temperature,three other conditions were optimized using response surface methodology. The optimum immobilization conditions were determined as follows：enzyme activity of free β-fructofuranosidase solution 900 U/mL and pH 6.6 for 4 h immobilization at room temperature,and the yield of fructooligosaccharides using immobilized β-fructofuranosidase under these conditions was 58.16%.

关 键 词：Β-D-呋喃果糖苷酶 离子交换法 固定化 响应面法 

分 类 号：Q814.2[生物学—生物工程]