碳源对枯草芽孢杆菌产α-淀粉酶的影响  

Effect of carbon sources on activity of α-amylase by B.subtilis

作　　者：李刚[1] 孙俊良[1] 葛晓虹[1] 梁新红[1] 

机构地区：[1]河南科技学院食品学院,河南新乡453003

出　　处：《食品与机械》2010年第5期13-14,18,共3页Food and Machinery

基　　金：河南省重点科技攻关项目(编号:102102210192);河南省教育厅自然科学研究项目(编号:2010A550004)

摘　　要：研究可溶性淀粉、蔗糖、葡萄糖3种主要碳源对枯草芽孢杆菌产α-淀粉酶活力、细菌浊度及其发酵培养过程中培养基pH值变化的影响。结果表明,枯草芽孢杆菌在摇床发酵试验中,当发酵时间超过54h酶活力基本达到最高且保持不变。以可溶性淀粉为碳源时最佳,枯草芽孢杆菌产α-淀粉酶酶活力最高为(114.5±2.3)U/mL,细胞浊度为2.342±0.023;其次是葡萄糖和蔗糖,最高酶活分别为(75.6±2.1),(72.2±1.2)U/mL,细胞浊度分别为2.515±0.031,2.421±0.044。可见枯草芽孢杆菌产生α-淀粉酶受淀粉的诱导,受蔗糖及葡萄糖的阻扼作用。The addition of glucose,sucrose and starch to the chemically defined medium,which was devised for use in the growth,pH and α-Amylase induction studies.As a result,the inoculated flask was incubated on a rotary shaker,the activity ofα-Amylase reached the highest point on 54h.When starch was used as a carbon source,the maximum activity ofα-amylase reached（114.5±2.3）U/mL,the cell density was 2.342±0.023.Then when glucose,sucrose were respectively used as a carbon source,the activity ofα-amylase were（75.6± 2.1）U/mL,（72.2±1.2）U/mL,the cell density of them were 2.515±0.031,2.421±0.044.Starch was shown to stimulateα-Amylase production by B.subtilis,whereas glucose,sucrose was shown to inhibit that.

关 键 词：碳源 枯草芽孢杆菌 Α-淀粉酶 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]