烟草中非挥发性有机酸的甲酯化条件优化及定量分析  

Methyl Esterification Optimization and Quantitative Analysis of the Non-Volatile Organic Acids in Tobacco

作　　者：张迎春[1] 蔡冰[2] 郭国宁[2] 李丹[2] 王承明[1] 

机构地区：[1]华中农业大学食品科学技术学院/农业部食品安全评价重点开放实验室,武汉430070 [2]湖北中烟工业有限责任公司烟用植物应用研究重点实验室,武汉430070

出　　处：《华中农业大学学报》2010年第6期788-793,共6页Journal of Huazhong Agricultural University

基　　金：国家烟草专卖局2007年重大专项基金项目(110200701003)资助

摘　　要：采用气相色谱-质谱法对15个不同产区的烟丝中苹果酸、富马酸、油酸等8种非挥发性有机酸进行定量分析并对甲酯化时间和甲酯化试剂的用量进行优化。试验表明,烟丝中非挥发性有机酸甲酯化的最佳条件为：酯化时间50 min,温度70℃,酯化试剂为V三氟化硼∶V甲醇=3∶7,在此条件下非挥发性有机酸的回收率为79.50%~101.35%,相对标准偏差为3.34%~7.32%。Eight kinds of non-volatile organic acids including malic acid,fumaric acid,9-octadecenoic acid and so on in cut tobacco from fifteen different planting areas were determined by GC/MS. The methyl esterification conditions of non-volatile organic acids were optimized. The results showed that the optimal time, temperature of methyl esterification was 50 min and 70 ℃. The optimal volume proportion of boron trifluoride to methanol, RSD of the method, the recovery of non-volatille organic acid was 3 ： 7, 3.34%-7. 32%,and 79.50%-101.35 %,respectively.

关 键 词：烟草 非挥发性有机酸 气相色谱-质谱 甲酯化 

分 类 号：Q657.63[生物学—生物物理学]