产葡萄糖异构酶工程菌工业发酵条件模拟研究  

STUDIES ON PRODUCTION CONDITIONS OF ENGINEERED STRAIN PRODUCING D-GLUCOSE ISOMERASE

作　　者：贺家明 赵祥颖 崔涛[2] 伍传金[2] 

机构地区：[1]山东省食品发酵工业研究设计院,济南250013 [2]中国科学技术大学生物系,合肥230026

出　　处：《微生物学通报》1999年第2期93-95,共3页Microbiology China

基　　金：国家高技术研究发展计划项目!863

摘　　要：高效表达葡萄糖异构酶的大肠杆菌工程菌Ｋ３８／ｐＧＰＩ－２，ｐＴＫＤ－ＧＩ菌株，在玉米浆培养基中能高效合成葡萄糖异构酶，观察了细菌生长的细胞浓度（ＯＤ）、ｐＨ和酶产生的动态变化。玉米浆培养基成本低、制备工艺简单，在５０Ｌ发酵罐中酶活力为１４３ｕ／ｍＬ，比在ＬＢ培养基中高约１０倍。用超声波破碎细胞液作酶源吸附于大孔阴离子交换树脂制成固定化葡萄糖异构酶、其酶活力达到１０２００ｕ／ｇ（干）。Studies indicate that D-glucose isomere (GI) could be synthesized in corn steep liquor byengineered E.coli strain K38 / pGPI-2, pTKD-GI. We beve also determined the optical density (OD),PH and activity of GI during fermentation. Com steep liquor medium has advantages in low cost,simplicity in technological process and high production GI. The production of GI reaches 143u/ mlin 50L fermentor which is ten times as large as that of LB medium. The broken cell solution wasable to prepare the immobilized GI adsorpted by the large pore anion exchange resin. The activity ofthe immobilized GI was 10200u / g dry.

关 键 词：工程菌 葡萄糖异构酶 玉米浆 工业发酵条件 

分 类 号：TQ925.5[轻工技术与工程—发酵工程]