氮源对里氏木酶合成木聚糖酶的影响  

INFLUENCES OF NITROGEN SOURCES ON XYLANASE SYNTHESIS BY TRICHODERMA REESEI

作　　者：洪枫[1,2] 陈琳[1,2] 余世袁[1,2] 沈兆邦[1,2] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]南京林业大学

出　　处：《林产化工通讯》1999年第3期7-10,共4页Journal of Chemical Industry of Forest Products

摘　　要：分别以（ＮＨ４）２ＳＯ４、酵母浸膏及蛋白胨为产酶氮源制备木聚糖酶。试验结果表明，酵母浸膏的产酶效果最好，其次是（ＮＨ４）２ＳＯ４。当以酵母浸膏为氮源产酶时，酶活力最高达到２５．４７ＩＵ／ｍＬ，酶得率和酶产率分别为３６３８．６ＩＵ／ｇ木聚糖和８４９０．０ＩＵ／Ｌ·ｄ，酶活力分别是以（ＮＨ４）２ＳＯ４及蛋白胨为氮源时的１．５倍和２．In this study, (NH4)2SO4, yeast extract and peptone have been investigated as nitrogen sources respectively for xylanase production by a strain of Trichoderma reesei. The results showed that the nitrogen sources had a marked effect on the activity of xylanase produced by T. reesei. Compared with (NH4)2SO4 and the peptone, the yeast extract was the best nitrogen source to produce xylanase. When the yeast extract was used as the nitrogen source, the highest activity, 25.47 IU/mL, was obtained with 7 g/L xylan as a substrate, with the yield and productivity being 3638.6 IU per gram of xylan and 8490.0 IU/Ld, separately. The xylanase activity was 1.5 times as high as that obtained with (NH4)2SO4, and 2.0 times as high as that prepared with peptone as the nitrogen source.

关 键 词：氮源 木聚糖酶 半纤维素酶 里氏木霉 

分 类 号：Q556[生物学—生物化学]