响应面优化磷脂酶A1催化酯化制备甘油二酯  

Optimization of production of diacylglycerol by esterification catalyzed by phospholipase A1 using response surface methodology

作　　者：王丽丽[1] 汪勇[1,2] 曹茜[1] 胡长鹰[1] 赵谋明[2] 黄惠华[2] 

机构地区：[1]暨南大学食品科学与工程系,广州510632 [2]华南理工大学轻工与食品学院,广州510641

出　　处：《中国油脂》2010年第11期40-43,共4页China Oils and Fats

基　　金：国家"863"计划项目(2010AA101505);粤港关键领域重点突破项目(2009A020700003);广东省科技计划项目(2009B080701063)

摘　　要：以大豆油脂肪酸和甘油为原料,经磷脂酶A1(Lecitase Ultra)催化酯化制备甘油二酯。利用响应面法优化试验条件,研究反应温度、加酶量、底物摩尔比值(甘油与脂肪酸摩尔比值)、反应时间对酯化率的影响。得出的最佳反应条件为:反应温度40℃,加酶量90 U/g,底物摩尔比值2.2,反应时间12 h。最佳条件下平均酯化率81.12%。反应混合液经过静置分层,采用分子蒸馏法除去油层中未反应的游离脂肪酸和副产物甘油单酯,得到甘油二酯产品,产品得率为51.32%,产品甘油二酯含量为72.62%。Production of diacylglycerol by esterification of fatty acid with glycerol catalyzed by phospholipase A1（Lecitase Ultra） was investigated.The optimum conditions including reaction temperature,enzyme dosage,molar ratio of substrates and reaction time were studied using response surface methodology（RSM）.The optimum conditions obtained by RSM were as follows： reaction temperature 40℃,enzyme dosage 90 U/g,molar ratio of glycerol to fatty acid 2.2∶1 and reaction time 12 h.Under these conditions,the esterification ratio was 81.12%.After settled to separate into two layers,the unreacted free fatty acid and byproduct of monoacylglycerol of the oily layer of reaction mixture were removed by molecular distillation to obtain diacylglycerol product with a yield of 51.32% and diacylglycerol content of 72.62%.

关 键 词：甘油二酯 响应面法 磷脂酶A1 酯化 

分 类 号：TQ641[化学工程—精细化工] Q559[生物学—生物化学]