2005年中国食源性单核细胞增生李斯特菌毒力基因分布  

Virulent gene prevalence of foodborne Listeria monocytogenes in China in 2005

作　　者：杨洋[1] 付萍[1] 郭云昌[1] 裴晓燕[1] 刘秀梅[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所 ,北京100021

出　　处：《中华预防医学杂志》2010年第12期1097-1101,共5页Chinese Journal of Preventive Medicine

基　　金：基金项目：“十一五”国家科技支撑计划（2006BAK02A15）

摘　　要：目的 了解中国食源性单核细胞增生李斯特菌（LM）毒力基因分布情况.方法 78株食源性LM分离自2005年中国内地13个省（市、自治区）的生肉、熟食、水产品和蔬菜等食品.采用PCR对LM的两个毒力岛LIPI-1（包括prfA、plcA、hly、mpl、actA、plcB等6种毒力基因）和LIPI-2（包括inlA、inlB等2种毒力基因）及毒力相关基因iap进行检测.结果 prfA基因检出率为87.2%（68/78）,plcA、actA、plcB基因检出率均为98.7%（77/78）,hly基因检出率97.4%（76/78）,mpl基因检出率87.2%（68/78）,inlA基因检出率92.3%（72/78）,inlB基因检出率100%（78/78）,iap 基因检出率98.7%（77/78）.在21株毒力基因缺失株中,7株毒力基因缺失株菌出现了2种或2种以上毒力基因缺失现象.分离自熟食的菌株的毒力基因缺失率为31.3%（10/32）,分离自生肉的菌株的毒力基因缺失率为16.1%（5/31）,分离自蔬菜的菌株的毒力基因缺失率为36.4%（4/11）,分离自水产品的菌株的毒力基因缺失率为50%（2/4）,差异无统计学意义（x2=3.721,P＞0.05）.毒力基因型1型菌株为优势菌株.结论 78株食源性LM的毒力基因除了inlB外,其余毒力基因都存在不同程度缺失现象,LIPI-1 中毒力基因普遍缺失,毒力基因缺失具有多样性.Objective To study the virulent gene prevalence of foodbome Listeria monocytogenes （LM） isolated from China. Methods 78 LM isolates derived from raw meat, cooked food,aquatic products and vegetables of 13 provinces and cities. LM isolates were investigated for prevalence of virulence genes （LIPI-1 （prfA, plcA, hly, mpl, actA, plcB）; LIPI-2 （inlA, inlB）, and iap） by PCR method. Results 87.2% （68/78） of the isolates were prfA positive,98. 7% （77/78） of the isolates were plcA, actA and plcB positive ,97.4% （76/78） of the isolates were hly positive, 87.2% （68/78）of the isolates were mpl positive,92. 3% （72/78） of the isolates were inlA positive,100% （78/78）of the isolates were inlB positive,98. 7% （77/78） of the isolates wereiap positive. Among 21 virulent gene negative isolates,there was 7 isolates lack of two or more virulence genes. The rate of virulence genes deletion isolates from cooked meat was 31.3%（10/32） ,the rate of virulence genes deletion isolates from raw meat was 16. 1% （5/31）,the rate of virulence genes deletion isolates from vegetables was 36. 4% （4/11） and rate of virulence genes deletion isolates from seafood was 50% （2/4）. No significant difference was found （x2 = 3. 721, P 〉 0. 05）. The virulence gene array-1 strains were dominant among these isolates. Conclusion Among 78 LM isolates,prevalent of virulent genes were different except inlB, virulence genes of LIP-1 were deleted prevalently among isolates, virulence gene deletion patterns were diverse.

关 键 词：毒力 基因 聚合酶链反应 利斯特菌 单核细胞增生 食品 

分 类 号：R686[医药卫生—骨科学]