尿素包合法纯化孔石莼不饱和脂肪酸  

Purification of unsaturated fatty acids from Ulva pertusa Kjellm by urea adduction

作　　者：楼乔明[1] 丛培旭[1] 徐杰[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品工业科技》2010年第12期160-161,共2页Science and Technology of Food Industry

基　　金：国家科技支撑计划(2008BAD94B04)

摘　　要：用Folch法提取孔石莼总脂,经皂化后酸化制得脂肪酸,再对脂肪酸进行尿素包合,使其中饱和脂肪酸与不饱和脂肪酸分离,从而提高不饱和脂肪酸的含量。在脂肪酸:尿素:无水乙醇比为1:2:20,温度为4℃,包合12h的条件下进行尿素包合结晶,效果较好,不饱和脂肪酸含量由72·43%增加到96·91%,其中十六碳四烯酸由13·63%增加到22·92%,增加68·16%,十八碳四烯酸由20·99%增加到38·82%,增加84·95%。The total lipid of Ulva pertusa Kjellm was extracted using Folch method,saponificated and acidificated to obtain the fatty acids.The saturated fatty acids and unsaturated fatty acids were separated by the method of urea adduction,thus improved the content of unsaturated fatty acids.The results of the experiment were preferable at the ration of fatty acids,urea and ethanol（1：2：20）,temperature（4℃）,adduction time（12h）.The content of unsaturated fatty acids was raised from 72.43% to 96.91%,and content of hexadecatetraenoic acid raised from 13.63% to 22.92%,stearidonic acid from 20.99% to 38.82%,and increased 68.16% and 84.95%,respectively.

关 键 词：孔石莼 尿素包合 纯化 不饱和脂肪酸 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]