黑曲霉固态混菌发酵苹果渣生产多酶生物饲料  

Production of bio-feed enriched of enzymes from apple pomace in solid state fermentation by Aspergillus niger

作　　者：庄童琳[1] 李虎 郭凤霞[1] 孙中涛[3] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]泰安嘉元生物工程公司,山东泰安271000 [3]山东农业大学生命科学学院,山东泰安271018

出　　处：《食品工业科技》2010年第12期171-175,共5页Science and Technology of Food Industry

基　　金：山东省科技攻关计划(2005GG1109002)

摘　　要：苹果渣是一类产量巨大而目前尚未充分利用的农业废弃物资源,其综合利用不仅可产生巨大的经济效益,而且还可以减少环境污染,具有显著的社会效益。本文采用两株黑曲霉对苹果渣进行固态混菌发酵生产多酶生物饲料,同时提高蛋白质与氨基酸含量,改善其营养价值。研究结果表明,采用苹果渣棉粕1:1(w/w)、(NH4)2SO41%(w/w)、KH2PO40·1%(w/w)为最佳培养基,黑曲霉F2与F3最佳配比为2:1(w/w),接种量0·4%(w/w),30℃恒温培养48h,蛋白酶和纤维素酶活力分别为3674U/g和1207U/g,果胶与单宁的降解率分别为99·0%和66·1%,同时,蛋白质与氨基酸的含量提高,可溶性糖含量降低,营养价值得到全面的改善。The objectives of this work were to produce bio-feed enriched of enzymes,biodegrade pectin and tannins,the main anti-nutritional factors in apple pomace,and to improve the nutritional level of the fermented substrate.Activities of proteinase and cellulase were 3674U/g and 1207U/g,and the biodegradation rates of pectin and tannins were 99.0% and 66.1%,respectively,when the optimum medium,the mixture of apple pomace and cottonseed powder（1：1,w/w）,supplemented with 1%（w/w）（NH4）2SO4 and 0.1%（w/w）KH2PO4,was inoculated with 0.4%（w/w）of Aspergillus niger F2 and F3（2：1,w/w）and incubated at 30℃ for 48h in solid state fermentation.The utilization of apple pomace in the paper was the most efficient biological treatment and could become a model for the value addition of similar wastes.

关 键 词：苹果渣 多酶生物饲料 抗营养因子 蛋白酶 纤维素酶 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]