CD81 Inhibits the Proliferation of Astrocytes by Inducing G_0/G_1 Arrest In Vitro  

作　　者：马俊芳 刘仁刚 彭会明 周洁萍 李海朋 

机构地区：[1]Department of Neurology,Tongji Hospital,Huazhong University of Science and Technology [2]Department of Anatomy,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2010年第2期201-205,共5页华中科技大学学报（医学英德文版）

基　　金：supported by a grant from the National Natural Sciences Foundation of China (No. 30570568)

摘　　要：Astrocytes play a major role in the reactive processes in response to neuronal injuries in the brain. Excessive gliosis is detrimental and can contribute to neuronal damage. CDS1 （TAPA）, a member of the tetraspanin family of proteins, is upregulated by astrocytes after traumatic injury to the rat central nervous system （CNS）. To further understand the role of CD81 in the inhibition of astrocytes, we analyzed the effects of a CD81 antibody, on cultured rat astrocytes. The results indicated that the effect worked in a dose-dependent manner with certain dosage range. It, however, reached a dosage equilibrium at a high dosage. Furthermore, anti-CD81 antibody remarkably inhibited the proliferation of astrocytes after incubation with astrocytes for different periods of time and the effect presented a time-dependent fashion. However, anti-CDS1 antibody substantially inhibited the proliferation of astrocytes at low density and middle density but slightly inhibited the proliferation of as- trocytes at high density, suggesting that the effect was positively correlated with the proliferative ability of astrocytes. Finally, the cell cycle of astrocytes exposured to anti-CD81 antibody was arrested in S phase at the initial stage and at G0/GI phase over time. These findings indicated that CD81 exert significant inhibitory effect, dose-dependently and time-dependently, on the proliferation of astrocytes and the effect is positively correlated with the proliferative capability of astrocytes.Astrocytes play a major role in the reactive processes in response to neuronal injuries in the brain. Excessive gliosis is detrimental and can contribute to neuronal damage. CDS1 （TAPA）, a member of the tetraspanin family of proteins, is upregulated by astrocytes after traumatic injury to the rat central nervous system （CNS）. To further understand the role of CD81 in the inhibition of astrocytes, we analyzed the effects of a CD81 antibody, on cultured rat astrocytes. The results indicated that the effect worked in a dose-dependent manner with certain dosage range. It, however, reached a dosage equilibrium at a high dosage. Furthermore, anti-CD81 antibody remarkably inhibited the proliferation of astrocytes after incubation with astrocytes for different periods of time and the effect presented a time-dependent fashion. However, anti-CDS1 antibody substantially inhibited the proliferation of astrocytes at low density and middle density but slightly inhibited the proliferation of as- trocytes at high density, suggesting that the effect was positively correlated with the proliferative ability of astrocytes. Finally, the cell cycle of astrocytes exposured to anti-CD81 antibody was arrested in S phase at the initial stage and at G0/GI phase over time. These findings indicated that CD81 exert significant inhibitory effect, dose-dependently and time-dependently, on the proliferation of astrocytes and the effect is positively correlated with the proliferative capability of astrocytes.

关 键 词：CD81 ASTROCYTES PROLIFERATION inhibition cell cycle TETRASPANINS 

分 类 号：R741[医药卫生—神经病学与精神病学]