北五味子红色素的纯化及抗氧化活性研究  

Purification and in vitro antioxidant activity of Schizandra Chinensis red pigment

作　　者：冯昕[1] 王吉中[1] 何培新[1] 严安心[1] 呼玉侠 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002 [2]上海奇泓生物科技有限公司郑州分公司,河南郑州450013

出　　处：《郑州轻工业学院学报（自然科学版）》2010年第5期28-30,共3页Journal of Zhengzhou University of Light Industry:Natural Science

基　　金：中国科学院知识创新工程重要方向项目(KSCXZ-YW-N-46-02-04)

摘　　要：提取了北五味子红色素,研究了该色素在D101大孔树脂上的动态吸附和洗脱工艺条件,并测定了红色素的体外抗氧化活性.结果表明,纯化后的红色素色价是未纯化的3.24倍;优化的动态吸附和洗脱条件为:吸附流速2mL/min,洗脱流速2mL/min,洗脱剂用量为4倍柱床体积.纯化后的色素,其清除羟自由基的能力和还原能力普遍高于未纯化色素,且抗氧化能力高低与色素浓度一致.The red pigment was extracted from Schizandra Chinensis.The technological conditions of dynamic adsorption and elution process of red pigment in D101 macroporous resin were studied.The antioxidative activity of red pigment in vitro was determined.The results showed that the color scale of purified pigment was 3.24 times higher than that of crude pigment.The better dynamic adsorption and adsorption conditions were those of feeding rate 2 mL/min,elute rate 2 mL/min and elute volume was 4 times higher than that of bed volume.The ability to clear the hydroxyl radical and the reducing power of purified pigment were higher than that of unpurified pigment,and was correspondent with the concentrations of pigments.

关 键 词：北五味子 红色素 纯化 抗氧化活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]