一株碱性蛋白酶产生菌的分离与应用  

Separation of a Alkali Protease-producing Strain and Its Application

作　　者：刘向宇[1] 郭华[1] 罗林[1] 

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410128

出　　处：《现代食品科技》2010年第12期1326-1329,共4页Modern Food Science and Technology

基　　金：湖南农业大学稳定人才项目(09WD36)

摘　　要：从湖南耒阳哲桥镇茶籽油榨油坊内采集土壤样品,分离筛选出能耐受茶皂素并产生碱性洗涤用蛋白酶的菌株。通过对筛选出的菌株进行初步形态鉴定、蛋白酶活力测定和污布去污效果比较,挑选出一株符合要求的菌株,命名为SJP-5。该菌株为革兰氏阳性产芽孢杆菌,无荚膜,能耐受茶皂素,产生碱性蛋白酶,其酶活力为150 U/mL,将其添加到清洗剂中并测定去污能力,试验表明:污布去污值Ri达到18,是平行菌株去污力的2倍。A G＋ strain of Bacillus,named SJP-5,was isolated from the soil around an oil mill in Zheqiao Town,Leiyang,Hunan Province.It showed alkaline protease-producing capability with its enzyme activity being of 150 U/mL.The decontamination ability of a detergent added with this strain showed that the Ri was of 18,which was 2 times higher than the detergents added with other strains.

关 键 词：油茶皂素 碱性蛋白酶 芽孢杆菌 

分 类 号：TQ925.2[轻工技术与工程—发酵工程]