LC-MS/MS法分析清爽型黄酒中的嘌呤含量  

LC-MS/MS Analysis of Purine Content in Light Chinese Rice Wine

作　　者：夏小乐[1] 夏梅芳 杨海麟[1] 张玲[1] 张斌 王武[1] 

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]南通如皋白蒲水明楼黄酒有限公司,江苏如皋226500

出　　处：《现代食品科技》2010年第12期1399-1402,共4页Modern Food Science and Technology

摘　　要：嘌呤是引起痛风的关键因素,检测黄酒中的嘌呤含量具有重要意义。本研究建立了一种采用液相色谱串联质谱法(LC-MS/MS)测定黄酒中嘌呤含量的方法。结果表明,清爽型黄酒中鸟嘌呤为0.67 mg/L,次黄嘌呤为0.62 mg/L,腺嘌呤为0.82 mg/L,总含量为2.12 mg/L。LC-MS/MS法样品加标回收率在98.2%-100.6%之间,相关系数大于0.995,相对标准偏差为3.8%-5.2%。A new LC-MS/MS method was developed to determine purine content in light Chinese rice wine.It was found that the contents of Guanine,Hypoxanthine and Adenine in qingshuang Chinese rice wine were 0.67 mg/L,0.62 mg/L and 0.82 mg/L,respectively.And the total purine content was 2.12 mg/L.The recovery of the samples was in the range of 98.2%~100.6%,and the R and RSD values were of 0.995 and 3.8%~5.2%,respectively.

关 键 词：清爽型黄酒 嘌呤 LC-MS/MS 

分 类 号：TS262.4[轻工技术与工程—发酵工程]