利用荧光假单胞菌固定化细胞生产2-酮基-D-葡萄糖酸  

Production of 2-Keto-D-Gluconic Acid Using Immobilized Pseudomonas fluorescens AR4

作　　者：陈萍[1] 苗晓燕[1] 张筱梅[1] 周强[2] 孙文敬[2,3] 

机构地区：[1]保定学院生化系,河北保定071000 [2]百勤异VC钠有限公司,江西德兴334221 [3]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品科学》2010年第21期258-261,共4页Food Science

基　　金：江苏大学科研启动基金项目(08JDG029);江西省主要学科学术和技术带头人培养计划项目(2008DD00600);江西省科技支撑计划项目(赣财教[2008]147号)

摘　　要：以海藻酸钠为载体固定荧光假单胞菌(Pseudomonas fluorescens)AR4,考察固定化细胞发酵生产2-酮基-D-葡萄糖酸的条件。结果表明:利用5.0%海藻酸钠制备的固定化细胞颗粒稳定性好,可重复利用7次;发酵培养基中玉米浆的最适质量浓度为0.75g/100mL,固定化细胞的适宜发酵温度为30～36℃;在适宜的培养条件下,固定化细胞与游离细胞的2-酮基-D-葡萄糖酸产量及糖酸转化率基本相同,分别可达13.5g/100mL和90.0%左右。The cells of Pseudomonas fluorescens AR4 were immobilized by sodium alginate,and the optimization of fermentation conditions for producing 2-keto-D-gluconic acid by immobilized cells were investigated.The results showed that 5.0% of sodium alginate was the optimal carrier for immobilizing Pseudomonas fluorescens AR4 to give a high yield,and the immobilized cells could be reused for 7 times.The optimal concentration of corn steep liquor in fermentation medium was 0.75 g/100 mL,and fermentation temperature was between 30 ℃ and 36 ℃.Under the optimal conditions,immobilized cells and free cells could give similar yield and conversion rate of 13.5 g/100 mL and 90.0%,respectively.

关 键 词：固定化细胞 荧光假单胞菌 2-酮基-D-葡萄糖酸 海藻酸钠 发酵 

分 类 号：TQ814.2[化学工程]