生淀粉糖化酶高产菌株诱变选育  

Mutating of raw starch-digesting glucoamylase strain

作　　者：孙子羽[1] 迟乃玉[1] 李兵[1] 王宇 张庆芳[1] 

机构地区：[1]大连大学生物工程学院,辽宁大连116622 [2]大连轻工业大学生物与食品工程学院,辽宁大连116034

出　　处：《大连大学学报》2010年第6期75-77,共3页Journal of Dalian University

摘　　要：以细菌Aeromonas.sp.CNY01为出发菌株,通过紫外(UV)和硫酸二乙酯(DES)诱变,获得产酶较高的菌株CNY01-27-13,酶活达到98.42 U/mL,比原始菌株酶活提高了110.52(,菌株经过9次传代,酶活较稳定。A mutant strain CNY01-27-13 with high yied of raw starch-digesting glucoamylase was isolated from protoplast of Aeromonas.sp.CNY01 treated by UV and DES.The raw starch-digesting glucoamylase activity of the strain raised to 98.42 U/mL,which was 110.52 % higher than the enzyme activity of protoplast strain.The enzyme activity of the mutant strain was very steady cultured by nine times.

关 键 词：生淀粉糖化酶 紫外诱变 DES诱变 突变株 

分 类 号：X172[环境科学与工程—环境科学]