产β-1,3-葡聚糖酶海洋细菌的筛选及其酶学性质研究  

Isolation,Identification and Enzymatic Properties of a Marine Bacterial Strain with High Production of β-1,3-glucanase

作　　者：刘长江[1] 金桥[1,2] 宋月[2] 李伟[2] 佟长青[2] 张彦龙[3] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110161 [2]辽宁省水产品加工及综合利用重点开放实验室,大连水产学院食品工程学院,辽宁大连116023 [3]黑龙江大学生命科学与工程学院,黑龙江哈尔滨150080

出　　处：《水产科学》2010年第12期703-707,共5页Fisheries Science

基　　金：辽宁省海洋与渔业厅科研计划项目(200915);大连市科技局项目(2008J22JH015);辽宁省教育厅资助项目(2009A163);大连水产学院科研项目(017050)

摘　　要：依据从海水中分离、筛选得到的-β1,3葡聚糖酶活较高的菌株SY-3之形态、生理及生化特性,初步鉴定为气单胞菌。该菌发酵产生的粗酶液经过硫酸铵沉淀、透析除盐后,对其酶学性质进行了初步研究。经测定,SY-3所产-β1,3葡聚糖酶的最适作用温度、pH分别为30℃、7.5。通过Lineweaver-Burk做图,得到其米氏常数(Km)为1.21 mg/ml。K+、Na+和Ca2+对酶促反应有促进作用,而Mg2+、Mn2+、Cu2+和Zn2+等有不同程度的抑制作用。A new bacterial strain SY-3 was isolated from marine water using classical selective enrichment,Congo red dye and polysaccharides from Auricularia auricular as the sole carbon source.The bacterium was identified as Aeromonas sp.SY-3 based on the phenotypical and biochemical properties.The β-1,3-glucanase was isolated from the broth of strain SY-3 by precipitation of（NH4）2SO4 and dialysis,and its activity was optimized at pH 7.5 and 30 ℃.The β-1,3-glucanase had apparent Km values of 1.21 mg/ml by Lineweaver-Burk plot,and the activity was enhanced by K＋,Ca2＋ and Na＋,while inhibited by Zn2＋,Mn2＋,Cu2＋ and Mg2＋.

关 键 词：β-1 3葡聚糖酶 海洋细菌 酶学性质 

分 类 号：Q939.99[生物学—微生物学]