噬菌体检测食源性致病菌的研究进展  

Research Progress of the Use of Bacteriophage to Detect Foodborne Pathogenic Bacteria

作　　者：李萌[1] 王静雪[1] 林洪[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2010年第23期439-446,共8页Food Science

基　　金：国家自然科学基金项目(31071540)

摘　　要：食源性致病菌是影响食品安全的主要因素之一。传统的检测手段已经不能满足食品安全快速检测的要求,食源性致病菌的检测技术面临着由耗时、费力的传统方法向省时、简便的现代检测技术转变的挑战。噬菌体作为细菌的天敌,具有结构简单、专一性强、分布广泛、繁殖速度快的特点。现今已有很多噬菌体检测食源性致病菌的方法,这些方法方便、快速、高度特异性,特别是当检测费用成为重要的考虑因素时,更有优势。本文对噬菌体检测食源性致病菌的技术原理及其应用进行分类综述和分析。Foodborne pathogenic bacteria have a significant impact on food safety. Traditional detection techniques are not sufficient to achieve a satisfactory detection results in short time period. Novel methods with rapidity, sensitivity and specificity are highly desired. Bacteriophages are virus-infecting bacteria, which are widespread in environment and have the characteristic of simple structure, high specificity and rapid propagation. Currently, many phage-based methods have been developed for inexpensive, fast and sensitive detection for foodborne pathogenic bacteria. In this paper, the principles and applications of phage-based methods are reviewed.

关 键 词：噬菌体 致病菌 检测方法 

分 类 号：Q939.48[生物学—微生物学] TS201.3[轻工技术与工程—食品科学]