马氏珠母贝高F值寡肽体外抗氧化研究  

Preliminary study on the antioxidant activity and protective effects on L02 cell injury of pinctada martensii high fischer ratio oligopeptides

作　　者：郑惠娜[1,2] 章超桦[1,2] 吉宏武[1,2] 秦小明[1,2] 

机构地区：[1]水产品深加工广东普通高校重点实验室,广东湛江524088 [2]广东海洋大学食品科技学院,广东湛江524088

出　　处：《食品与机械》2010年第6期63-66,共4页Food and Machinery

基　　金：国家科技支撑计划课题(编号:2007BAD29B09);现代农业产业技术体系建设专项资金资助(编号:贝类;47);广东海洋大学引进人才基金(编号:0912259)

摘　　要：研究马氏珠母贝高F值寡肽(PHFP)的体外抗氧化及其对H2O2诱导L02肝细胞损伤的保护作用。结果表明:马氏珠母贝高F值寡肽对·OH、DPPH·及O2-·均具有一定的清除能力。在浓度为10mg/mL时,对3种自由基的清除率分别达到了60.8%、50.8%和44.2%。剂量为1mg/mL的高F值寡肽使H2O2诱导损伤L02肝细胞的半数致死剂量LD50由损伤模型组的0.618mmol/L提高到0.737mmol/L,提高了19.3%。The free radical scavenging activity of PHFP and the protective effects of PHFP on L02cells injury induced by H2O2 were studied.The results indicated that in chemical reaction system,the scavenging ability of PHFP（10mg/mL）for three kind of free radical ·OH、DPPH·and O2-·was 60.8%,50.8%and 44.2%respectively.And when the L02cells were treated with 1mg/mL PHFP,the LD50of H2O2changed from 0.618mmol/L to 0.737mmol/L,increased by 19.3%.

关 键 词：马氏珠母贝 高F值寡肽 抗氧化活性 

分 类 号：TQ461[化学工程—制药化工]