金属离子对羊血超氧化物歧化酶活性的影响  

Effect of metal ions on activities of SOD extracted from sheep blood

作　　者：杨学山[1] 杨孝朴[1] 吴兵[1] 杨志杰[1] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《食品工业科技》2011年第1期89-91,共3页Science and Technology of Food Industry

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2010-03)

摘　　要：以离子交换层析法制备的羊血SOD(Superoxide dismutase)为原料,加入不同浓度的Na+、K+、Ba2+、Ca2+、Mg2+、Zn2+、Cu2+、Fe2+等金属离子,25℃保温4h后,利用邻苯三酚自氧化法测定SOD酶活力,并计算相对酶活力。结果表明:不同金属离子在不同浓度下对羊血SOD酶活的影响不同。Na+对SOD酶活影响不大;K+、Ca2+、Fe2+在体外对羊血SOD具有激活作用,且Fe2+作用明显,相对酶活力可达378.98%;Ba2+、Mg2+在低浓度下对SOD影响不大,在高浓度下有一定的激活作用,但不同浓度激活效果不同;Zn2+、Cu2+在体外对羊血SOD表现有抑制作用,但二者的抑制程度不同。The activities of SOD extracted by ion-exchange chromatography from sheep-blood enzyme solution were determined by means of pyrogallo autoxidation method under the different concentrations of Na＋ , K＋ , Ba2＋ , Ca2＋ ,Mg2＋ ,Zn2＋, Cu2＋, Fe2＋ at 25℃ for 4h.The results showed that different concentrations of metal ions had different influence on sheep blood SOD activity. Na ＋ had little effect on SOD activity. K ＋ , Ca2 ＋ and Fe2 ＋ had an activation on the activity of SOD in vitro, especially Fe2＋ could add the relative enzyme activity of SOD up to 378.98%.Ba2＋ , Mg2＋ had little effect on activity of SOD at low concentrations,whereas the activation was detected at high concentrations.Zn2. and Cu2＋ inhibited the activition of SOD in vitro.

关 键 词：超氧化物歧化酶 金属离子 酶活力 

分 类 号：TS201.1[轻工技术与工程—食品科学]