黏质沙雷氏菌产几丁质酶二步发酵工艺的优化  

Optimization of Chitinase Production by Serratia marcescens with Two-Step Fermentation

作　　者：施腾鑫[1] 贺淹才[1] 刘嘉[1] 舒静波[1] 周娟[1] 

机构地区：[1]华侨大学工业生物技术研究所,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2011年第1期67-71,共5页Journal of Huaqiao University(Natural Science)

基　　金：福建省自然科学基金资助项目(C04010011)

摘　　要：采用二步发酵法,研究黏质沙雷氏菌的产几丁质酶发酵条件.在二步发酵产酶的过程中,通过单因素法优化得到二步发酵培养基中最适碳源、氮源的质量分数和菌龄,同时选取发酵时间、初始pH值、接种菌体量和装液量4个因子进行正交试验.最终得到的最优条件:胶体几丁质质量分数为0.8%,酵母粉质量分数为1.0%,菌龄为12h,发酵时间为72h,初始pH值为8.5,菌体干质量为90mg,装液量为20mL.在此条件下,酶活力可达17.020μkat.L-1.另外,在二步发酵工艺中添加0.1%纤维素,酶活力可提高至18.804μkat.L-1,比一步发酵提高1.27倍.Two-step fermentation was used to investigate the fermentation conditions of chitinase production by Serratia marcescens.The optimum bacterial age and the concentrations of carbon source and nitrogen source in the two-step fermentation were obtained through single factor method.At the same time,fermentation time,initial pH value,bacterial dose and loadage were selected as objects with orthogonal design.The chitinase activity reached 17.020 μkat·L-1 under the optimized condition： colloidal chitin 0.8%,yeast extract 1.0%,baceria age 12 h,incubation time 72 h,initial pH 8.5,bacteria dry weight 90 mg,loadage 20 mL in 250 mL conical flask.By adding 0.1% cellulose to two-step fermentation,activity of chitinase was 18.804 μkat·L-1 that was 1.27 times higher than that of one-step fermentation.

关 键 词：黏质沙雷氏菌 几丁质酶 二步发酵 一步发酵 优化 

分 类 号：Q814.1[生物学—生物工程] TQ920.6[轻工技术与工程—发酵工程]