具有溶栓活性的重组乳酸乳球菌的构建  

Construction of recombinant Lactococcus lactis with fibrinolytic activity

作　　者：余凤云[1] 冯浩[1] 许芳[1] 闫达中[1] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023

出　　处：《中国酿造》2011年第1期100-103,共4页China Brewing

基　　金：湖北省教育厅中青年项目(Q200718003)

摘　　要：利用PCR方法从纳豆芽孢杆菌(Bacillus subtilis subsp.natto)基因组DNA中扩增纳豆激酶原基因,构建了纳豆激酶原基因的表达载体pFY002,转化Lactococcus lactis NZ9000,得到活性表达纳豆激酶的重组菌Lactococcus lactis NZ9000/pFY002。利用纤维蛋白平板法检测其溶栓活性,并对诱导剂乳链菌肽(NisinA)的浓度、温度、诱导时间对重组菌产酶的影响进行探究。Pro-nattokinase gene was amplified by PCR from Bacillus subtilis subsp, natto genomic DNA, which was cloned into pGT-19 vector and then sequenced. The recombinant plasmid pFY002 was constructed by inserting pro-nattokinase gene into expression vector pNZ8048 and was transformed into Lactococcus lactis NZ9000. The fibrlnolytic activity was analyzed after the expression of gene was induced by nisin A. The optimum conditions including nisinA concentration, temperature and induction time in the engineering L. lactis were further investigated.

关 键 词：纳豆激酶 乳酸乳球菌 克隆表达 

分 类 号：Q933[生物学—微生物学]