日落黄和柠檬黄人工抗原的合成与鉴定  

Synthesis and Identification of the Artificial Antigens for Sunset Yellow and Tartrazine

作　　者：林菲[1] 刘丽强[1] 匡华[1] 彭池方[1] 宋珊珊[1] 胡金伟[1] 胥传来[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品科学》2011年第1期124-126,共3页Food Science

摘　　要：通过综合分析日落黄和柠檬黄的化学结构,构建半抗原,采用多种偶联方式将其与载体蛋白偶联,制备完全抗原。通过采用紫外光谱扫描和液相色谱-质谱联用技术对合成产物进行了鉴定和分析,结果表明,日落黄的半抗原合成不够理想,偶联比率为6;而柠檬黄的完全抗原经鉴定偶联成功,偶联比率是12。该实验结果可为制备可特异性地识别柠檬黄的抗体提供参考。This study was aimed to develop suitable complete antigens based on either sunset yellow or tartrazine,two pigment compounds.Based on the chemical structural characteristics of the target molecules,we used various activation methods including succinic anhydride and mixed anhydride to acquire complete antigens.Bovine serum albumin（BSA） was served as the carrier protein to couple with the haptens.The results from ultra-violet spectral and high perform liquid chromatography-mass spectrometry analyses showed that the immunogen based on tartrazine was synthesized successfully.The coupling ratio of tartrazine to the carrier protein was 12,while that was only 6 for sunset yellow-BSA conjugate.The tartrazine-BSA conjugate prepared in this study can be used to induce the generation of the specific antibodies against tartrazine in animals.

关 键 词：日落黄 柠檬黄 人工抗原 合成 

分 类 号：TS202.3[轻工技术与工程—食品科学]