杜仲粕中桃叶珊瑚苷的分离和纯化  

Separation and Purification of Aucubin from Eucommia ulmoides Seed Meal

作　　者：李辉[1,2] 文赤夫[1,2] 李亚男[1] 彭玉丹[1] 汪兰[1] 张永康[1,2] 

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]吉首大学植物资源保护与利用省级重点实验室,湖南吉首416000

出　　处：《食品科学》2011年第2期32-35,共4页Food Science

基　　金：湖南省教育厅优秀青年项目(06B076)

摘　　要：为研究大孔树脂对桃叶珊瑚苷的吸附与解吸性能及分离纯化效果,通过静态吸附与解吸实验从12种大孔树脂中筛选高性能树脂,用树脂进行柱层析分离目标产物并优化分离条件。结果表明:大孔树脂SIPI-7、HZ-820和S-8对目标分子既有高的吸附率,又能有效脱附;当用HZ-820型树脂进行柱层析分离纯化桃叶珊瑚苷时,优化分离条件为上样量0.5g/g(以生药质量:树脂质量计)、乙醇-水-冰醋酸混合溶剂(50:50:3,V/V)为洗脱剂、流速1.0mL/min。在优化条件下,收集含产物浓度高的流分,经浓缩和重结晶后所获产品中桃叶珊瑚苷纯度高达96.82%,且具有可观的产品得率。The objective of this study was to develop a macroporous resin adsorption method for separating and purifying aucubin in Eucommia ulmoides seed meal extracted into 80%aqueous ethanol solution.Twelve kinds of macroporous resins were compared for differences in their adsorption and desorption performances towards aucubin,and the results indicated that macroporous resins SIPI-7,HZ-820 and S-8 had better abilities to adsorb and desorb aucubin,among which,HZ-820 was considered to be the best resin due to the highest desorption rate.The optimal conditions for the adsorption and desorption of HZ-820 were found to be：sample loading,0.5 g/g（weight ratio of Eucommia ulmoides seed meal extracted to HZ-820 resin）;desorpion solvent,50%aqueous ethanol solution containing 3%acetic acid at a flow rate of 1.0 mL/min.The high aucubin eluate fractions obtained under such conditions were pooled,condensed and recrystallized into a considerable amount of product containing 96.82%of aucubin.

关 键 词：桃叶珊瑚苷 分离纯化 大孔树脂 杜仲粕 

分 类 号：TS201.1[轻工技术与工程—食品科学]