细胞转化液γ-氨基丁酸的精制  

Purification of Gamma-Aminobutyric Acid from the Supernatant after S.salivarius subsp.thermophilus Y-2 Transformation Reaction

作　　者：杨胜远[1] 陆兆新[2] 别小妹[2] 吕凤霞[2] 

机构地区：[1]韩山师范学院生物系食品与发酵工程研究所,广东潮州521041 [2]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品科学》2011年第2期244-247,共4页Food Science

基　　金：国家"863"计划项目(2007AA10Z357);广东省自然科学基金项目(8452104101001546);国家星火计划项目(2008GA780032);韩山师范学院科研基金项目

摘　　要：对Streptococcus salivarius subsp.thermophilus Y-2细胞转化液GABA的纯化进行探讨,通过等电点沉淀和732强酸阳离子交换树脂处理。结果表明:GABA的总回收率达到84.13%;纯化的GABA的外观为白色粉末状固体,GABA含量(97.81±0.67)%,灰分(0.449±0.002)%,干燥质量损失率(1.79±0.06)%,铅、砷和汞的含量分别为(0.4093±0.0001)、(0.0511±0.0001)、(0.0950±0.0000)mg/kg,纯化的GABA质量符合食品添加剂标准。该方法可高效纯化细胞转化液中的GABA。The concentrated supernatant after S.salivarius subsp.thermophilus Y-2 transformation reaction rich in gamma-aminobutyric acid（GABA）was adjusted to pH 3.22 for precipitate L-Glu,followed by injection into 732 type strong acidic cation exchange resin column and elution with 0.15 mol/L NH3·H2O.After freeze-drying,GABA was obtained as a white solid powder,with 84.13%recovery.The powder contained（97.81±0.67）%of GABA,（0.449±0.002）%of ash,and exhibited a dry weight loss of（1.79±0.06）%,and the contents of Pb,As and Hg in it were（0.4093±0.0001）,（0.0511±0.0001）mg/kg and（0.0950± 0.0000）mg/kg,respectively.Moreover,its quality was in accordance with relevant food additive standards.Thus,the purifica-tion strategy is highly efficient.

关 键 词：纯化 Γ-氨基丁酸 生物转化 唾液链球菌嗜热亚种 

分 类 号：TS202.3[轻工技术与工程—食品科学]