不同反应条件对酶改性胡萝卜纤维的影响  

Effect of Different Reaction Conditions on Carrot Fiber Properties Modified by Cellulase

作　　者：刘欢[1] 张晓明[2] 王新伟[1] 马中苏[1] 

机构地区：[1]吉林大学生物与农业工程学院,长春130025 [2]吉林农业大学园艺学院,长春130118

出　　处：《中国农业科技导报》2010年第6期81-85,共5页Journal of Agricultural Science and Technology

基　　金：国家863计划项目(2008AA10Z308);吉林省科技发展计划项目(20060717)资助

摘　　要：以胡萝卜渣中提取的胡萝卜纤维(carrot fiber,CF)为原材料进行酶改性,研究纤维素酶浓度和反应时间对CF的平均长度、平均宽度、长宽比、还原糖浓度和可及度的影响,以及纤维素酶改性前后CF平均长度和平均宽度的分布情况。结果表明,随着纤维素酶浓度和反应时间的增加,CF的平均长度、平均宽度和长宽比减小;还原糖浓度和可及度增加。从节约纤维酶用量和时间的角度考虑,当纤维素酶浓度为0.4 mg/mL和反应时间为2 h时,平均长度、平均宽度、长宽比、还原糖浓度和可及度最佳。Carrot fiber（CF） made from carrot pomace was modified by cellulose and then used to investigate the effect of cellulose content and reaction time on average length,average width,L/W,sugar content and accessibility of CF.The composite action of cellulase modified CF was also analyzed.The result showed that with the increasing of cellulase concentration and reaction time,the average length,average width,L/W decreased,while the sugar content and accessibility increased.Considering quantum conservation and reaction time of cellulose used,0.4 mg/mL and 2 h were optimal for the average length,average width,L/W,sugar content and accessibility of CF.

关 键 词：胡萝卜纤维 纤维素酶 改性 反应条件 

分 类 号：TQ92[轻工技术与工程—发酵工程]