产脂肪酶真菌的选育及酶学性质研究  

Screening and breeding of lipase-producing fungi and a study of the lipase properties

作　　者：李珍[1] 程子彰[1] 徐振杰[1] 胡容[1] 杨洲平[1] 黄乾明[1] 

机构地区：[1]四川农业大学生命科学与理学院,雅安625014

出　　处：《菌物学报》2011年第1期60-68,共9页Mycosystema

基　　金：四川省教育厅重点科研项目(No.08ZA053);四川农业大学科技创新基金项目(No.00731200)

摘　　要：从富含油脂土壤中筛选出一株产碱性脂肪酶酶活达6.40U/mL的真菌菌株,经显微形态及ITS序列分析鉴定为产黄青霉Penicillium chrysogenum,该菌株命名为Penicillium chrysogenum J23。该菌的最佳产酶培养条件为:蔗糖1.0%、蛋白胨2.0%、橄榄油1.0%、MgSO4·7H2O0.05%、接种量1.0%、初始pH9.0、摇床转速200r/min、30℃培养48h。其所产脂肪酶的最适反应温度与pH分别为33℃和7.5,在pH6.0-10.0酶具有良好的稳定性,在50℃处理2h仍可保持30%以上的酶活力,50mmol/L的Ca2+、Mg2+、K+分别对酶有较强激活作用,而50mmol/L的Fe2+、Mn2+、Cu2+、Pb2+、Li2+对酶则有不同程度的抑制作用。Penicillium chrysogenum J23 lipase activity reached maximal level of 6.40U/mL. Maximum enzyme production was observed in the medium containing sucrose 0.1%,peptone 2.0%,olive oil 1.0%,and MgSO4·7H2O 0.05%. The medium was inoculated with an initial volume of 1.0% seed culture,agitated at 200r/min and incubated for 48h. The optimal temperature and acidity for lipase to decompose lipid was found at 30℃ and pH9.0,respectively. Lipase characterization showed that its maximal activity was observed at 33℃ and pH7.5. Moreover,the lipase was highly stable under the pH value ranging from 6.0 to 10.0,and indicated 30% of total activity at 50℃. The results also showed that this kind of lipase was activated by high concentration （50mmol/L） of common detergent ions Ca^2＋,Mg^2＋,K^＋,but inhibited by Fe2＋,Mn^2＋,Cu^2＋,Pb^2＋,Li^2＋.

关 键 词：真菌 脂肪酶 鉴定 酶学性质 产黄青霉 

分 类 号：Q93[生物学—微生物学]