超高效液相色谱法同时测定冷饮中12种食品添加剂  

Simultaneous Determination of Twelve Kinds of Food Preservatives in Cold Drink by Ultra-high Performance Liquid Chromatography

作　　者：蔡志斌[1] 张英[1] 刘丽[1] 

机构地区：[1]深圳市龙岗区疾病预防控制中心,广东深圳518172

出　　处：《实用预防医学》2011年第1期138-141,共4页Practical Preventive Medicine

摘　　要：目的建立超高效液相色谱法同时测定冷饮中安赛蜜、糖精钠、阿斯巴甜、苯甲酸、山梨酸、脱氢乙酸、柠檬黄、苋菜红、胭脂红、日落黄、亮蓝、咖啡因12种食品添加剂水平的方法。方法样品用水直接稀释后,用带有二极管阵列检测器的超高效液相色谱仪测定,色谱柱为WATERS ACQUITY UPLC BEH C18(粒径1.7μm,柱内径×长为2.1 mm×50mm),流动相为乙腈+磷酸盐溶液(调pH=5.0),梯度洗脱,流速0.2ml/min,波长:230nm。结果 12种食品添加剂16min内完全分离,质量浓度线性范围为0.10～50.00mg/L,最低检出质量分数为0.5mg/kg,回收率为91.6%～103.6%,相对标准偏差<3%。结论该方法简单、快速、灵敏度高,适用于冷饮中12种食品添加剂水平的同时测定。Objective To establish a simultaneous test method for the determination of twelve kinds of food preservatives in cold drink by ultra-high performance liquid chromatography（UPLC）. Methods After diluting,the samples were tested by UPLC with a diode array detector and then separated by WATERS ACQUITY UPLC BEH C18 column（particle size： 1.7μm,length multiply diameter of column： 2.1 mm ×50mm）.Phosphate buffer（pH=5.0） and acetonitrile were used as the mobile phase.Gradient elution analysis was conducted.The flow rate was 0.2 ml/min.The detective wavelength was 230 nm. Results Twelve kinds of sweeteners could be well separated within 16 min.The linear range was 0.10～50.00 mg/L.The minimum detectable concentration was 0.5 mg/kg.The average recovery rate was 91.6%～103.6%.RSD was below 3%. Conclusions This method is simple and quick,and with high sensitivity.It is an effective way to determine twelve kinds of food preservatives in cold drink simultaneously.

关 键 词：甜味剂 防腐剂 色素 咖啡因 冷饮 超高效液相色谱法 

分 类 号：R-331[医药卫生]