利用糖蜜发酵生产生物降解塑料PHB的研究  

PRODUCTION OF POLY(3-HYDROXYBUTYRATE) BY ALCALIGENES LATUS GROWN ON MOLASSES

作　　者：翁维琦[1] 易祖华[1] 黄和容[1] 陈琦[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学通报》1999年第5期311-314,共4页Microbiology China

摘　　要：由Ａｌｃａｌｉｇｅｎｅｓｌａｔｕｓ经单菌落分离，从中筛选到一株高效利用糖蜜产聚羟基丁酸（ＰＨＢ）的优良菌株１０１８。在６Ｌ台式发酵罐（Ｎ.Ｂ.Ｓ）中，进行了该菌利用甜菜糖蜜和甘蔗糖蜜积累ＰＨＢ的分批补料培养的研究。结果表明，培养５４ｈ左右发酵液中细胞干重达７０～８５ｇ／Ｌ，ＰＨＢ含量占细胞干重的６０％～７０％，生产强度１．０ｇＰＨＢ／Ｌ／ｈ以上；发酵液经非有机溶剂提取制得ＰＨＢ产品，纯度９５％，提取收率８０％以上。Production of poly (3-hydroxybutyrate ) (PHB ) by fed-batch culture of Alcaligenes latus 1018 wasinrestigated using beet and cane molasses in a 6L fermentor. In 54 hours the cell concentration and polymercontent of dry cell weight reached 70-85g / L and 60%-70%, respectively, and the productivity was up to 1.0gPHB / L / h. An aqueous digestion process was used for recovering the product from A latus with the recoveryof more than 80% and the purity of PHB was 95%.

关 键 词：糖蜜 发酵 肥大产碱杆菌 PHB 生物降解塑料 

分 类 号：TQ324.9[化学工程—合成树脂塑料工业] Q939.1[生物学—微生物学]