双抗夹心ELISA方法检测食品中单核细胞增生李斯特氏菌  

Detection of Listeria monocytogenes in Food by Sandwich ELISA

作　　者：段霞[1] 黄欣[2] 黄岭芳[1] 魏华[1] 赖卫华[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌市西湖区疾病预防控制中心,江西南昌330025

出　　处：《食品科学》2010年第24期272-276,共5页Food Science

基　　金：科技部中小企业创新基金项目(10C26223601934)

摘　　要：采用0.5%福尔马林灭活的单核细胞增生李斯特氏菌作为免疫原免疫日本大耳兔,获得抗单核细胞增生李斯特氏菌多克隆抗体,并以此多克隆抗体作为捕获抗体,以抗单核细胞增生李斯特氏菌InternalinA(InlA)单克隆抗体作为检测抗体,建立快速、特异的检测该菌的双抗夹心ELISA方法。结果表明:该方法对单核细胞增生李斯特氏菌纯培养液的最低检测量为1.7×105CFU/mL。在检测食品样品的实验中,食品样本对本方法干扰较小,运用选择性增菌液进行前增菌可提高该方法的准确性。A rapid, specific and sensitive sandwich enzyme-linked immunosorbent assay （ELISA） was developed to detect L. monocytogenes in food samples, which using polyclonal antibody as the capture antibody and anti-Internalin monoclonal antibody as the detection antibody. Anti-Listeria monocytogenes polyclonal antibody was prepared by using 0.5% formalin inactivated L. monocytogenes as antigen and Japanese rabbits as the host animal. The detection limit was 1.7×105 CFU/mL in pure culture. Results showed that food samples had limited interference to the detection, while pre-enrichment using selective enrichment broth could significantly increase the detection limit and sensitivity.

关 键 词：单核细胞增生李斯特氏菌 多克隆抗体 单克隆抗体 双抗夹心ELISA方法 

分 类 号：TS207.3[轻工技术与工程—食品科学]