高效液相色谱法对绿茶中茶多酚含量的测定  

HPLC Determination of Polyphenols in Green Tea

作　　者：侯冬岩[1] 回瑞华[1] 李铁纯[1] 刁全平[1] 魏威[1] 刘晓媛[1] 

机构地区：[1]鞍山师范学院化学系,辽宁鞍山114007

出　　处：《食品科学》2010年第24期305-307,共3页Food Science

基　　金：辽宁省教育厅科学技术基金项目(20331079)

摘　　要：采用高效液相色谱法测定绿茶中茶多酚的含量。在色谱柱为KromasilC18、流动相为乙酸:甲醇:N,N-二甲基甲酰胺:水=1:1:40:58(V/V)、色谱柱温30℃、进样量10μL、检测器为紫外检测器、检测波长280.0nm的色谱条件下对绿茶中茶多酚含量进行测定。结果表明:回归方程为Y=1×10-7X-0.0036(r=0.9995),方法变异系数小于3.04%,回收率为99.16%～100.60%;6种绿茶样品茶多酚含量为62.07～110.86mg/g,说明产地不同,绿茶中茶多酚的含量相差较大。本方法简便、准确,适合绿茶中茶多酚含量的分析。The polyphenol contents in green tea were determined by high performance liquid chromatography method, using the mobile phase consisting of acetic acid, methyl alcohol, N,N- dimethylformamide and warter （1：1：40：58, V/V）. The column temperature was 30 ℃, and the detection wavelength was 280.0 nm. The regression equation obtained was Y=1×10-7X-0.0036 （r =0.9995）. The coefficient of variation was less than 3.04%, and the recovery was 99.16%-100.60%. The result indicates that the proposed method is simple , accurate and feasible for determination of polyphenols in the green teas.

关 键 词：绿茶 高效液相色谱法 茶多酚 测定 

分 类 号：O657.32[理学—分析化学]