羊乳中掺入牛乳的间接ELISA定量检测  

Indirect ELISA for Detection and Quantification of Bovine Milk in Goat Milk

作　　者：薛海燕[1] 胡围围[1] 宋宏新[1] 韩燕[1] 杨战月 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021 [2]兴平市人民医院,陕西兴平713100

出　　处：《食品科学》2010年第24期370-373,共4页Food Science

基　　金：陕西省科学技术研究发展计划项目(2009K02-09);陕西省教育厅产业化培育项目(09JC19);陕西省教育厅自然科学专项(2010JK425)

摘　　要：制备抗β-酪蛋白多克隆血清,免疫吸收封闭与羊乳反应的抗体,获免疫吸收抗体用于乳样的间接ELISA检测中,建立羊乳中掺入牛乳成分的定量免疫学方法。实验表明,该间接ELISA法用于原乳检测时,掺入牛乳的百分含量与A450nm在4%～50%呈线性关系,该方法的最低检出量为4%。所建立的ELISA方法变异系数<5%,回收率在94%～105%之间,符合方法学要求,可用于牛乳掺假的定量检测。对灭菌乳的检测表明,热处理不改变β-酪蛋白与抗体反应的特性,方法还可用于经热加工的乳品检测中。An indirect enzyme-linked immunosorbent assay （ELISA） was developed for the detection and quantification of bovine milk adulteration in goat milk. The polyclonal antibodies were modified by mixed them with goat milk for the assay. The absorbance at 450 nm in indirect ELISA revealed a linear relationship with the concentration of adulterated bovine milk at the range of 4%-50%. Detection limit was 4% for mixed milk samples. The assay was characteristics of high reproducibility with intra- and inter-assay variation coefficients less than 5%. The recovery rate of spiked samples was in the range of 94%-105%. Therefore, the ELISA can successfully use to determine the adulteration of milk samples, which is suitable for developing a kit in routine inspection of milk.

关 键 词：多克隆抗体 酶联免疫吸附反应(ELISA) 羊乳掺假 牛乳 

分 类 号：Q51[生物学—生物化学]