过氧化氢酶高产菌株CE-2-A的发酵条件优化  

Optimization of fermentation conditions for the catalase production by Aeromonas sp. CE-2-A

作　　者：张增祥[1,2] 王伟[1] 王芳[1] 孙谧[1] 

机构地区：[1]海洋产物资源与酶工程实验室,中国水产科学研究院黄海水产研究所,青岛266071 [2]上海海洋大学食品学院,201306

出　　处：《渔业科学进展》2010年第6期95-103,共9页Progress in Fishery Sciences

基　　金：国家高技术研究发展(863)计划(2007AA091602);国家自然科学基金(41006119)共同资助

摘　　要：为了提高气单胞菌属菌株CE-2-A的过氧化氢酶产量,对其发酵条件进行优化。首先运用了单因子试验筛选出了发酵培养基的麦芽糖含量、牛肉膏含量、CaCl2和MgCl2含量,在此基础上采用Plackett-Burman设计法,对8种影响产酶的因素进行评价。试验结果表明,牛肉膏含量、起始pH、摇瓶装液量对菌株产过氧化氢酶的活力具有显著的影响。通过Box-Behnken试验考察了牛肉膏含量、起始pH、摇瓶装液量这3个主要因素对菌株所产过氧化氢酶活力的影响。发酵条件优化结果为装液量42.6ml、牛肉膏含量3.69g/L、起始pH9.1,采用优化后发酵培养条件进行摇瓶发酵培养,过氧化氢酶的酶活力达到2532.5U/ml,与优化前酶活力(1849.7U/ml)相比提高了36.9%。The effective three-phase statistical method was used to optimize the fermentation conditions for the catalase production by Aeromonas sp.CE-2-A.Firstly,the content of maltose,beef extract,calcium chloride and magnesium chloride in the fermentation medium was screened through single factor experiments.Based on the results of the first step,eight factors that affected the enzyme production were evaluated by using the method of Plackett-Burman design.It showed that the medium volume,beef extract content and original pH had great influence on the catalase activity of strain CE-2-A.The effects of three factors on the enzyme activity produced by the strain were determined by the Box-Behnken experiment.The results of fermentation optimization were as follows：medium volume 42.6 ml,beef extract content 3.69 g/L,original pH 9.1,and the enzymatic activity of catalase after optimization in shaking flasks was improved from 1,849.7 U/ml to 2,532.5 U/ml,a 36.9% increase compared with the initial fermentation conditions before the optimization.

关 键 词：气单胞菌 过氧化氢酶 发酵条件 响应面分析 

分 类 号：Q939.9[生物学—微生物学] TQ925[轻工技术与工程—发酵工程]