响应面法优化橡实淀粉生料发酵生产燃料酒精工艺  

Study on the optimum process of acorn starch uncooked fermentation for alcohol by RSM

作　　者：谢碧霞[1] 李安平[1] 田玉峰[1] 丁彦鹏[1] 崔富贵[1] 

机构地区：[1]中南林业科技大学,湖南长沙410004

出　　处：《中南林业科技大学学报》2010年第12期92-97,共6页Journal of Central South University of Forestry & Technology

基　　金：国家公益项目"野生木本淀粉种质资源评价与精加工技术研究"(200804033);湖南省科技计划项目"橡实淀粉生物乙醇关键技术研究"(2010NK3040)

摘　　要：运用单因素试验和响应面试验研究了生料发酵法制备酒精的工艺。结果表明:原料粉碎度为100目、α-淀粉酶添加量为9 U/g、糖化酶添加量为201 U/g、料水比为1?2.8、酸性蛋白酶添加量为36 U/g、发酵温度为36℃、发酵时间为72 h等工艺条件下,可获得最高的橡实淀粉酒精转化率。Through the research of single factor tests and response surface methodology（RSM） tests,the optimal fermentation conditions were summed up as follows：the particle size is 100 meshes,the ratio of material to water is 2.8,the addition of α-amylase is 9 U/g,the addition of glucoamylase is 201 U/g,the addition of acid protease is 36 U/g,the fermentation temperature is 36℃,the fermentation time is 72 h.

关 键 词：橡实淀粉 生料发酵 响应面法 工艺 

分 类 号：S792.18[农业科学—林木遗传育种]