响应面法优化生物酶法合成植物甾醇酯  

Applying Response Surface Methodology to Optimize the Lipase-catalyzed Synthesis of Phytosterol Ester

作　　者：王腾宇[1] 王铭义[2] 宋玉卿[1] 胡立志[1] 于殿宇[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]哈尔滨理工大学测控技术与通信工程学院,黑龙江哈尔滨150086

出　　处：《食品科学》2011年第4期59-63,共5页Food Science

基　　金：国家"863"计划项目(2010AA101503);黑龙江省教育厅科学技术研究项目(11551064)

摘　　要：采用Novozym 435脂肪酶催化大豆油与植物甾醇的酯交换反应,对其技术参数和条件进行探索。通过单因素与响应面分析,确定最佳工艺参数:反应温度101℃、酶用量1%、甾醇添加量5.2%、反应时间3h。在最佳条件下酯交换反应的转换率高达85.67%。Transesterification was conducted in soybean oil with phytosterol under the catalysis of Novozym 435 lipase. The optimum reaction conditions were obtained by single factor experiments and response surface analysis to be reaction between soybean oil and added phytosterol at a concentration of 5.2% for 3 h at 101 ℃ in the presence of 1% lipase. The rate of transesterification was 85.67% under these conditions.

关 键 词：植物甾醇 大豆油 酯交换反应 脂肪酶 

分 类 号：TQ645.98[化学工程—精细化工]