曲酸遗传毒性的研究  

Genetic Toxicity of Kojic Acid:Its Effects on Mice Mironucleus and DNA Damage in CHO-K1 Cells

作　　者：陈永红[1] 黄夏宁[2] 邹志飞[1] 谷康定[2] 王岚[1] 刘慧智[1] 

机构地区：[1]广东检验检疫技术中心,广东广州510623 [2]华中科技大学同济医学院公共卫生学院,湖北武汉430030

出　　处：《食品科学》2011年第3期228-232,共5页Food Science

基　　金：广州市科技计划项目(2007Z3-E0381);广东出入境检验检疫局科技计划项目(2005GDK56)

摘　　要：目的:研究曲酸遗传毒性及有关作用机制。方法:采用小鼠骨髓细胞微核实验检测染色体损伤。用不同质量浓度曲酸(0、500、1000、1500、2000、2500μg/mL)诱导CHO-K1细胞,单细胞凝胶电泳实验检测CHO-K1细胞DNA损伤。结果:1/2LD50、1/4LD50和1/8LD50不同剂量曲酸诱导昆明小鼠微核实验结果显示,各质量浓度受试组与阴性对照组差别无显著性。单细胞凝胶电泳结果显示:曲酸作用质量浓度为1000μg/mL,作用时间为6h时,即出现轻微DNA损伤;当曲酸作用质量浓度增大到2500μg/mL时,出现明显的DNA损伤。在24h内,或其他同一作用时间段,随着曲酸作用质量浓度增加,DNA损伤也呈增加趋势。实验未发现有明显的时间-效应关系。结论:体内小鼠骨髓细胞微核实验未观察到曲酸明显的遗传毒性,但体外实验高质量浓度曲酸在一定时间可导致DNA损伤。Objective： To investigate the genetic toxicity and its mechanisms of kojic acid. Methods： Bone marrow micronucleus test in mice and comet assay in CHO-K1 cells were used to examine DNA damage. KM mice were randomly divided into 5 groups for 5 males and 5 females in each group. The KM mice were administered with kojic acid at doses of 1/2, 1/4, and 1/8 fold LD50 for examining DNA damage. CHO-K1 cells were treated with kojic acid at the concentrations of 0, 500, 1000, 1500, 2000 μg/mL and 2500μg/mL for comet assay at 6, 12, 24 h and 48 h, respectively. Results： After treatment with kojic acid, the ratio of micronucleus in mice did not exhibit a significant increase, compared with the negative control. The slight DNA damage in CHO-KI cells was observed in kojic acid group at the concentration of at 1000 μg/mL after 6 h incubation. Severely DNA damage was also observed when kojic acid concentration was increased to 2500 μg/mL. However, no response-time relationship was observed. Conclusion： Kojic acid at the experimental conditions did not result in genetic toxicity in bone marrow cells of treated mice, but could induce DNA damage in vitro at high concentration level.

关 键 词：曲酸 传代细胞株 微核实验 单细胞凝胶电泳 

分 类 号：Q523[生物学—生物化学]