纤维素乙醇木聚糖酶的固体发酵工艺研究  

Studies on solid state fermentation of xylanase for cellulosic ethanol

作　　者：杨付伟 王林风 任建伟 赵子高 程远超 

机构地区：[1]河南天冠企业集团,河南南阳473000

出　　处：《可再生能源》2011年第1期23-26,共4页Renewable Energy Resources

基　　金：国家科技支撑计划(2007BAD66B01)

摘　　要：该研究立足于河南天冠企业集团纤维素乙醇项目,以酶解糖化工艺对木聚糖酶的需求为出发点,利用黑曲霉X06作为产酶菌株,采用固体发酵工艺,通过正交设计试验,优化了培养基配方和发酵控制工艺,最优方案:麸皮∶玉米芯=6∶4、硝酸铵4%、尿素1%、磷酸二氢钾0.4%、硫酸镁0.2%、初始含水量65%、初始pH=4.0、温度28℃、环境相对湿度70%、72 h酶活达到10 096.74 IU/g。这一方案在生产中得到进一步放大和优化,所生产的固体木聚糖酶应用在秸秆酶解工艺中,酶解液中木糖含量提高了66.9%。Based on the needs of xylanase in the cellulosic ethanol project of Henan Tianguan Group Co.,Ltd.,Using Aspergillus niger X06 as xylanase-producing strain,through orthogonal experiment,the optimal solution of solid state fermentation was obtained as follows： the wheat bran to corn cob ratio is 6：4,the NH4NO3 content is 4%,the CO（NH2）2 content is 1%,the KH2PO4 content is 0.4%,the MgSO4·7H2O content is 0.2%,the initial moisture content is 65%,the initial pH value is 4.0,the incubation temperature is 28 ℃,the relative humidity of the environment is 70%.The activity of xylanase could reach 10 096.74 IU/g in 72 h.This program has been amplified and further optimized in large-scale production.The production of solid xylanase has been used in straw hydrolysis process,and the xylose content increased by 66.9%.

关 键 词：木聚糖酶 固体发酵 生产工艺 纤维乙醇 

分 类 号：TK6[动力工程及工程热物理—生物能] TQ920.6[轻工技术与工程—发酵工程]