柠条叶蛋白碱性提取的响应面分析法优化  

Optimization of Leaf Protein Alkaline Extraction from Caragana microphylla Lam.by Response Surface Methodology

作　　者：刘杰[1] 贾士儒[1] 钟成[1] 李宾[1] 范利花[1] 

机构地区：[1]工业微生物教育部重点实验室天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2011年第1期1-4,共4页Journal of Tianjin University of Science & Technology

基　　金：国家科技支撑计划项目(2007BAK36B05-4);天津科技大学引进人才科研启动基金资助项目(20100413)

摘　　要：柠条是一种粗蛋白含量较高的灌木,而且富含全部的人体必需氨基酸.从丰富的柠条资源中提取蛋白对于部分缓解我国目前的蛋白质资源紧缺以及环境保护等问题具有积极作用;因此,通过Box-Behnken实验设计进行柠条叶蛋白碱性提取的响应面优化,得到了适当的模型,并确定了重要参数的最佳水平.根据系统给定的参数得到的预测值与实测值相当接近;柠条叶蛋白提取的最佳工艺条件为:NaOH溶液浓度0.12,mol/L,絮凝pH,2.9,温度75,℃.最终可得柠条叶蛋白浓缩物相对得率(RLPCY)和蛋白相对得率(RPY)分别为16.2%和41.5%.Caragana microphylla Lam.is shrub with high protein level and all of the essential amino acid.Extracting pro-tein from C.microphylla Lam.offers good alternative to partly eliminate protein shortage and improve environmental con-cerns in China.Leaf protein alkaline extraction was optimized by Box-Behnken design coupled with response surface metho-dologya,nd a regressive model was obtained.The predicted values matched well with the experimental data within the given experimental conditions.The optimal technological parameters were optimized as followc：oncentration of NaOH 0.12 mol/Lf,locculation of pH 2.9 and flocculation of temperature 75,℃.In optimized conditiont,he relative leaf protein concen-trates yield（RLPCY）and relative protein yield（RPY）of C.microphylla Lam.could reach 16.2% and 41.5% respectively.

关 键 词：柠条 叶蛋白 碱性提取 响应面法 

分 类 号：Q946.1[生物学—植物学]