硝基磺酚C-Cu(Ⅱ)-蛋白质-CTMAB体系的吸收光谱及其应用  

Study on Absorption Spectrum of Nitrosulfophenol C-Cu(Ⅱ)-Proteins-CTMAB System and its Application

作　　者：胡秋娈[1] 冯爱青[2] 李建涛[1] 

机构地区：[1]洛阳师范学院化学化工学院,河南洛阳471022 [2]洛阳师范学院生命科学系,河南洛阳471022

出　　处：《食品研究与开发》2011年第2期100-102,共3页Food Research and Development

基　　金：河南省科技攻关重点项目(102102110154);河南省教育厅自然科学基金(2011A150020)

摘　　要：红色的硝基磺酚C在pH2.52的Britton-Robinson缓冲介质中能与Cu2+反应生成稳定的蓝色配合物,其配合物与生物大分子蛋白质形成有色复合物,λmax=676 nm,比试剂本身红移120 nm,表观摩尔吸光系数7.48×105 L/(mol.cm),蛋白质在0～100μg/mL范围内遵循比尔定律,CTMAB存在,灵敏度提高35%。方法线性范围宽,选择性高基本无干扰,操作简便,重现性好,灵敏度比直接用硝基磺酚C光度法高7.6倍。用于卵蛋白的测定,结果满意。Red nitrosulfophenol C can react with proteins and Cu^2＋ to form complex in Britton-Robinson buffer solution at pH 2.52, which gives maximum absorption peak at 676 nm with 120 nm to red shift compared to that of nitrosulfophenol C. A molar absorptivity of 7.48×105 L/（mol·cm） and linear range of 0-100 μg/mL for protein were detemined. By adding surfactants to form quaternary, reaction system, the sensitivity increased by 35 %, and was 7.6 times that of binary reaction system. This method has been used for the detemination of the total protein in egg with satisfactory results.

关 键 词：铜(Ⅱ)-硝基磺酚C配合物 卵蛋白 吸光光度法 

分 类 号：TS201.2[轻工技术与工程—食品科学]