木瓜蛋白酶水解制备菲律宾蛤仔肽工艺研究  

On the preparation of peptides from Ruditapes philippinarum by papain

作　　者：曲敏[1,2] 宋月[2] 李伟[2] 孔亮[3] 冯叙桥[1] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110161 [2]辽宁省水产品加工与综合利用重点开放实验室大连海洋大学,辽宁大连116023 [3]近岸海洋环境科学与技术重点实验室大连海洋大学,辽宁大连116023

出　　处：《北京农学院学报》2011年第1期58-60,共3页Journal of Beijing University of Agriculture

基　　金：辽宁省教育厅资助项目(2009A163);大连市科技局项目(2008J22JH015)

摘　　要：利用壳聚糖为载体、戊二醛为交联剂固定化的木瓜蛋白酶,水解菲律宾蛤仔。通过正交试验对pH值、酶用量、酶解时间、温度等参数进行了优化。结果表明:在pH 6.0条件下,酶解菲律宾蛤仔的最佳工艺条件为固定化木瓜蛋白酶酶加量(E/S)1.8%、酶解时间9 h以及温度60℃,所得菲律宾蛤仔肽含量为60.4 mg。Chitosan was used as carrier and glutaraldehyde as a cross-linking agent for the immobilization of papain.They were applied in the preparation of hydrolysates from proteins of Ruditapes philippinarum.Some parameters such as pH,the amount of immobilized papain,time and temperature were optimized through orthogonal test.The results showed that optimal conditions of enzymolysis were 1.8% of immobilized papain,reaction time of 9 h and temperature 60 ℃ at pH 6.0.In addition,peptide recovery of the R.philippinarum was 60.4 mg.

关 键 词：壳聚糖 固定化 木瓜蛋白酶 菲律宾蛤仔 肽 

分 类 号：TS254.5[轻工技术与工程—水产品加工及贮藏工程]