20种多孔类真菌乙醇提取物体外抗氧化和抗肿瘤活性的比较研究  

Comparisons on Antioxidant and Anti-tumor Activity of Ethanol Extracts from 20 Polypores Fungi in vitro

作　　者：陈永强[1] 陈先晖[1] 孙勇[1] 缪倩[1] 王力[1] 蒋继宏[1] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《食品科学》2011年第5期27-31,共5页Food Science

基　　金：国家"863"计划项目(2007AA021506);江苏省高校自然科学重大基础研究项目(08KJA350001)

摘　　要：采用清除DPPH自由基的方法和Alamar Blue法比较20种多孔类真菌乙醇提取物抗氧化活性和对3株癌细胞体外增殖的影响,对它们的子实体和菌丝体乙醇提取物进行测定。结果表明:20种多孔菌子实体乙醇提取物清除自由基和抗肿瘤活性大部分都强于菌丝体乙醇提取物,其中有10株多孔类真菌子实体乙醇提取物质量浓度在5mg/mL时,自由基清除率超过70%;质量浓度在500μg/mL时,对人非小肺癌细胞NCI-H460和人肝癌细胞HepG2两株癌细胞抑制率在90%左右,而对耐药细胞株Bel-7402/5-Fu抑制率略低于正常细胞株。The antioxidant activities of ethanol extracts from the mycelia and fruit bodies of twenty polypores fungi were evaluated by 1,1-diphenyl-2-picrylhydrazyl（DPPH） free radical scavenging method.Meanwhile,these extracts were tested for their anti-tumor activities by Alamar Blue method.The results showed that the DPPH free radical scavenging and anti-tumor activities of the extracts from fruit bodies of each of these fungi were significantly higher than those of the extracts from mycelia.A DPPH radical scavenging rate of 70% was observed for the ethanol extract of fruit body at a concentration of 5 mg/mL,and the inhibition rates of the ethanol extract against two cancer cell lines such as non-small lung cancer cell NCI-H460 and human hepatoma cell HepG2 were both around 90% at 500 μg/mL.In addition,compared with the normal cell lines,a slightly low inhibition rate of the ethanol extract on drug-resistant cells such as Bel-7402/5-Fu was also observed.

关 键 词：多孔菌 抗氧化 DPPH自由基 抗肿瘤活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]