响应面法优化Bacillus thuringiensis ZJOU-010产壳聚糖酶  

Optimization of Chitosanase Production from Bacillus thuringiensis ZJOU-010 by Response Surface Methodology

作　　者：陈静[1] 陈小娥[1] 方旭波[1] 余辉[1] 

机构地区：[1]浙江海洋学院食品与药学学院医学院,浙江舟山316000

出　　处：《食品科学》2011年第5期176-181,共6页Food Science

基　　金：浙江省自然科学基金项目(Y305119);浙江省优先主题农业项目(2008C12029);浙江海洋学院科研启动基金项目

摘　　要：目前,利用壳聚糖酶降解壳聚糖已成为生产功能性壳寡糖的首选途径。本研究在单因素试验的基础上,通过响应面法结合中心组合设计优化了Bacillus thuringiensis ZJOU-010产壳聚糖酶的培养条件。结果表明:当虾壳粉质量浓度为44.96g/L、(NH4)2SO4质量浓度为1.48g/L、pH值为6.78时,B.thuringiensis ZJOU-010的壳聚糖酶活力达到4.25U/mL。优化后获得的实验值与模型的预测值(4.16U/mL)基本相符,且较优化前的酶活力(3.59U/mL)提高了18.47%。研究表明,利用生物催化技术以虾加工企业的副产物——虾壳粉为唯一碳源和氮源生产壳寡糖是可行的。Chitooligosaccharides exhibit a broad range of potential applications in the fields of biomedicine,pharmaceuticals,food,cosmetics and agriculture.In the present study,based on the results from single factor tests,response surface methodology（RSM） combined with central composite design was used to optimize cultivation conditions of Bacillus thuringiensis ZJOU-010 for producing chitosanase.The optimal conditions were achieved to be shrimp shell powder（SSP） concentration of 44.96 g/L,（NH4）2SO4 concentration of 1.48 g/L,and pH of 6.78.Under the optimal conditions,the chitosanase activity from Bacillus thuringiensis ZJOU-010 was 4.25 U/mL.The obtained value of chitosanase activity was in good agreement with the predicted value（4.16 U/mL）.Meanwhile,18.47% improvement on chitosanase activity after the optimal cultivation was achieved,when compared with the chitosanase activity of 3.59 U/mL before optimization.These results indicated that oligosaccharides could be produced using shrimp processing by-products as the carbon and nitrogen sources.

关 键 词：壳寡糖 虾壳粉 壳聚糖酶 优化 响应面 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]