非水介质中脂肪酶催化合成蔗糖-6-乙酸酯  

Lipase-catalyzed Synthesis of Sucrose-6-acetate in Nonaqueous Medium

作　　者：王勍[1] 郑璞[1] 倪晔[1] 孙志浩[1] 

机构地区：[1]江南大学生物工程学院,工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与发酵工业》2010年第12期20-24,共5页Food and Fermentation Industries

基　　金：国家重点基础研究发展计划“973”(No.2003CB716008);国家自然科学基金(No.20473049)项目

摘　　要：研究了以蔗糖为起始原料,在非水介质中脂肪酶催化合成蔗糖-6-乙酸酯。通过对反应介质、脂肪酶和酰基供体的筛选、转酯反应条件的优化得出,在DMSO/叔丁醇体系中,固定化脂肪酶Lipozyme TLIM能较好地催化乙酸乙烯酯和蔗糖进行转酯反应;反应的较佳条件为:DMSO/叔丁醇体积比1∶4,蔗糖与乙酸乙烯酯的摩尔比1∶10,蔗糖浓度0.06 mmoL/mL,固定化脂肪酶浓度80 mg/mL,反应温度30℃,时间9 h,生成蔗糖-6-乙酸酯的产率为89.3%。并对产物进行了红外光谱及核磁共振分析,确证合成产物为蔗糖-6-乙酸酯。Synthesis of sucrose-6-acetate in non-aqueous medium from sucrose using lipase was studied.The transesterification conditions including reaction medium,acyl donor,enzyme,water content and temperature were investigated.Results showed that immobilized-lipase Lipozyme TLIM could effectively catalyzed sucrose and vinyl acetate to produce sucrose-6-acetate in the medium composing of DMSO and tert-butyl alcohol.A mole yield of 89.3% was reached when the transesterification was performed as follows;volume ratio of DMSO to tert-butyl alcohol 1：4,mole ratio of vinyl acetate to sucrose 10：1,concentration of sucrose 0.06 mmoL/mL,concentration of,enzyme 80 mg/mL,reaction temperature of 30 ℃ and reaction time of 9 h.The structure of the products was also characterized as sucrose-6-acetate by FTIR and NMR.

关 键 词：蔗糖-6-乙酸酯 非水介质 脂肪酶 酰基供体 转酯反应 

分 类 号：TS202.3[轻工技术与工程—食品科学]