高效液相色谱法测定饮料中甜味剂和防腐剂的含量  

Simultaneous Determination of Preservatives and Sweeteners in Drinks by HPLC

作　　者：杨理[1] 闫清华[2] 王新生[3] 

机构地区：[1]河南科技学院实验中心,河南省新乡市华兰大道453003 [2]新乡医学院生命科学技术系,河南省新乡市华兰大道453003 [3]河南科技学院新科学院,河南省新乡市华兰大道453003

出　　处：《光谱实验室》2011年第2期870-874,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：河南科技学院高层次人才资助项目(6037)

摘　　要：采用高效液相色谱-紫外检测器测定饮料中甜味剂和防腐剂的含量。色谱柱为EclipseXDB-C18(4.6mm×250mm,5μm),以甲醇∶0.02mol/L乙酸铵溶液(pH 6.0,30∶70,V/V)为流动相,柱温为25℃,流速为0.7mL/min,在230nm处检测。结果表明,各物质的线性回归方程相关系数r在0.9990—0.9998之间,回收率在98.40%—109.22%。该方法简便,灵敏,快速,可靠,重现性好,适用于质量检测。A method for the simultaneous determination of preservatives and sweeteners in drinks by high performance liquid chromatography was described.The experiments were carried out by EclipseXDB-C18（4.6mm×250mm,5μm） with methanol-0.02mol/L NH4Ac（30∶70,V/V,pH=6.0） as mobile phase at a flow rate 0.7mL/min and column temperature of 25℃.The UV detection wavelength was fixed at 230nm.The result indicated that the correlation coefficient of all subjects was between 0.9990 and 0.9998,the recovery was 98.40%—109.22%.The method is easy,sensitive,quick,reliable,repeatable and suitable for quality test.

关 键 词：高效液相色谱法 饮料 甜味剂 防腐剂 

分 类 号：O657.72[理学—分析化学]