基于毛细管区带电泳系统的南方水牛奶酪蛋白分析技术研究  

Analysis of Water Buffalo Caseins in South China by Capillary Zone Electrophoresis

作　　者：王丽娜[1] 李子超[1] 李昀锴[1] 徐明芳[1] 

机构地区：[1]暨南大学生命科学技术学院生物工程系,广东广州510632

出　　处：《分析测试学报》2011年第3期264-268,共5页Journal of Instrumental Analysis

基　　金：广东省科技计划资助项目(2009B011300003)

摘　　要：该文建立了一种毛细管区带电泳（CZE）分离南方水牛奶酪蛋白（CN）的方法，并对其进行定量分析。采用50．2cm×75μmi．d．的未涂层熔融石英毛细管柱，在运行电压为25kV，柱温为25℃，检测波长为214nm的条件下，研究了缓冲液体系、缓冲液浓度、pH、尿素浓度及进样时间等对水牛奶酪蛋白分离的影响。实验测得α—CN、β-CN、κ-CN在0．5～5．0g／L范围内线性关系良好，相关系数均不低于0．9980，加标回收率为98％～106％，酪蛋白各组分的保留时间、峰面积及峰高的相对标准偏差（RSD）分别不高于0．67％、3．0％及3．2％。该法简便、快速、准确，可用于水牛奶及其乳制品的质量控制和定量检测。A capillary zone electrophoresis （CZE） method was developed for the separation and quantitative analysis of water buffalo caseins. The effects of several parameters, including buffer system and concentration, pH value, urea concentration and injection time on the separation efficiency of water buffalo caseins were investigated on an uncoated fused-silica capillary column（ 50.2 cm ×75μm i. d. ） using separation voltage of 25 kV and detection wavelength of 214 nm at 25 ℃. Under the optimal conditions, the calibration curves were linear in the range of 0.5 - 5.0 g/L for α-CN, β-CN and κ-CN with correlation coefficients no less than 0. 998 0. The spiked recoveries of three main caseins were in the range of 98% - 106%. The relative standard deviations（RSDs） for migration time, peak area and peak height were no more than 0.67% , 3.0% and 3.2% , respectively. This method was simple, rapid and accurate, and could be used for the quality control and quantitative analysis of water buffalo milk and dairy products.

关 键 词：毛细管区带电泳 酪蛋白 水牛奶 定量分析 

分 类 号：O657.8[理学—分析化学] O629.73[理学—化学]