微生物发酵合成γ-聚谷氨酸的初步表征分析  

Analysised on γ-polyglutamic Acid's Characters with Method of Fermentation

作　　者：王风青[1] 梁金钟[1] 

机构地区：[1]哈尔滨商业大学食品工程学院,哈尔滨150076

出　　处：《食品工业》2011年第3期7-11,共5页The Food Industry

摘　　要：以一株γ-聚谷氨酸高产菌——枯草芽孢杆菌B-115为实验菌株,通过单因素试验对其发酵条件进行优化,结果显示:最佳发酵条件为初始pH6.5、接种量2%、装液量50 mL/250 mL、转速150 r/min、37℃培养84 h;γ-聚谷氨酸的产率从57.85 g/L提高为68.3 g/L。且通过薄层层析、红外检测等方式对其发酵产物进行初步表征分析。With a high productive poly γ-glutamic acid strain --Bacillus subtilis B-115, single-factor optimization of fermentation conditions were carried out. The results showed that the suitable fermentation conditions were as follows： inoculums level 2%, flask content 50 mL/250 mL, pH 6.5, temperature 37℃, rotation speed 150 r/min, and fermentation time 84 h. Under above conditions, the yield of 7-PGA increased from 57.85 g/L to 68.3 g/L. Besides, The product of fermentation was analysised by thin-layer chromatography, infrared detection and so on.

关 键 词：Γ-聚谷氨酸 枯草芽孢杆菌 培养条件 结构表征分析 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]