产胞外多糖的黒鲷肠道乳酸菌的分离鉴定及其生物学特性  

Isolation,identification and biological characteristics of an exopolysaccharide-producing lactic acid bacteria strain from intestinal tract of black porgy(Sparus macrocephalus)

作　　者：戴贤君[1] 章蔚[1] 刘明启[1] 黄晓林[2] 

机构地区：[1]中国计量学院生命科学学院,浙江杭州310018 [2]浙江省海洋水产养殖研究所,浙江温州325005

出　　处：《浙江大学学报（农业与生命科学版）》2011年第2期149-154,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省自然科学基金资助项目((Y307519);浙江省温州市科技计划资助项目(S20090047)

摘　　要：通过常规鉴定和16SrDNA序列分析，从黑鲷（Sparus macrocephalus）肠道中分离鉴定出1株明串珠菌属（Leuconostoc）乳酸菌菌株；该分离株对酸、胆盐、蛋白酶具有良好耐受性，但对温度耐受能力较差；分离株培养上清液对哈维氏弧菌（Vibrioharveyi）、溶藻弧菌（Vibrioalginolyticus）、副溶血弧菌（Vbrioparahaemolyticus）的抑菌圈直径为1 .12～1．22cm，不同培养时间的抑菌效果差异无统计学意义（P〉0．05）；分离株在培养24、32、40、48和56h时的胞外多糖质量浓度为167．24～184．45mg．L，不同取样时间的胞外多糖质量浓度差异无统计学意义（P〉0．05）；灌服胞外多糖20d后黑鲷血液的红细胞、白细胞数量和血红素浓度、淋巴细胞比率均显著升高（P〈0．05）．综上，该分离株为产胞外多糖的明串珠属乳酸菌，可作为开发海水鱼用微生态制剂的候选菌株．A strain of exopolysaccharide-producing lactic acid bacteria was isolated from intestines of black porgy, Sparus macrocephalus, and was identified as Leuconostoc lactis by conventional biochemical characteristics, 16S rDNA gene sequence analysis. Physiological-biochemical tests for the isolate showed that it had good tolerance to acid, bile salt and protease, but couldn＇ t live above 70℃. The culture supernatant of the isolate could inhibit the growth of Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus, and the inhibition zone diameters were 1.12-1.22 cm and had not significant difference （P〉 0.05） at different culture time of 24, 32, 40, 48, 56 h, The concentrations of exopolysaccharides in the culture medium were 167.24-184.45 mg ·L^-1 with no significant difference （P〉0.05） at the culture time of 24, 32, 40, 48, 56 h. The erythrocyte and leukocyte counts, hemoglobin concentration and lymphocyte ratio of black porgies increased significantly （P〈0. 05） after being fed orally exopolysaccharides from the isolate culture for 20 d. The results above indicate the isolate, Leuconostoc lactis can be a candidate probiotic strain in marine aquaculture.

关 键 词：黑鲷 明串珠属乳酸菌 胞外多糖 分离 鉴定 生物学特性 

分 类 号：Q939.6[生物学—微生物学]