游离态和固定态青霉素酰化酶对环境的敏感性  

SENSITIVITY OF PENICILLIN ACYLASE IN FREE AND IMMOBILIZED STATE TO ENVIRONMENTAL FACTORS

作　　者：杨海波[1] 虞星炬[1] 曲天明[1] 苏志国[2] 

机构地区：[1]中国科学院大连化学物理所,大连116023 [2]中国科学院化学冶金所,北京100080

出　　处：《中国医药工业杂志》1999年第10期439-440,479,共3页Chinese Journal of Pharmaceuticals

摘　　要：在优化的固定化条件下，通过戊二醛交联直接将青霉素酰化酶固定化。比较了环境因素对游离酶和两种固定化酶活性的影响，结果表明青霉素酰化酶固定化后，抗环境变化的能力明显提高。Penicllin acylase was directly immobilized with glutaraldehyde as the crosslinking agent under the optimal immobilizing conditions.Compared the effects of environmental factors on the activity of the free enzyme with that of two kinds of immobilized enzymes,the immobilized enzymes were found to increase their resistance to the changes of environment.The activity of immobilized enzyme cross linked with glutaraldehyde showed that it was more suitable for utilization on industrial scale.;

关 键 词：青霉素酰化酶 环境因素 酶活性 抗生素 酶法 

分 类 号：TQ465.91[化学工程—制药化工] TQ927[轻工技术与工程—发酵工程]