乙醇对简单节杆菌TCCC 11037生长和细胞性质的影响  

The Effects of Ethanol on Cell Growth and Cell Characteristics of Arthrobacter Simplex TCCC 11037

作　　者：王建锋[1] 骆健美[1] 宋妍[1] 刘洋[1] 郑宇[1] 王敏[1] 

机构地区：[1]天津科技大学生物工程学院工业微生物教育部重点实验室天津市工业微生物重点实验室,天津300457

出　　处：《化学与生物工程》2011年第3期66-69,共4页Chemistry & Bioengineering

摘　　要：研究了乙醇体积分数对简单节杆菌TCCC 11037生长和细胞性质的影响。结果表明,随着乙醇体积分数的增大,菌体的延滞期延长,生物量和比生长速率下降,当乙醇体积分数为6%时几乎不能观察到明显的菌体生长;菌体形态随着乙醇体积分数的增大出现较明显的改变,当乙醇体积分数为8%时,细胞完整性明显破坏,胞浆大量外泄;菌体的耗糖能力随着乙醇体积分数的增大受到明显抑制,菌体生长到32 h时,对照组和3%乙醇添加组中的葡萄糖浓度由9.8g.L-1分别下降到2.2 g.L-1和5.6 g.L-1,6%乙醇添加组几乎没有明显的糖耗。利用Rh123法和FDA荧光法分别测定了乙醇体积分数对菌体跨膜电位和通透性的影响。结果表明,随着乙醇体积分数的增大,跨膜电位下降,细胞膜通透性提高,当乙醇体积分数为3%和6%时,跨膜电位分别下降了14.14%和24.58%,细胞膜通透性分别增加了6.58%和18.37%。In this paper,effect of ethanol volume fraction on cell growth and cell characteristics of Arthrobacter simplex TCCC 11037 were studied.The results showed that cell biomass and specific growth rate declined and the lag phase prolonged with ethanol volume fraction increased.When 6%（volume fraction） ethanol were added,the measurable cell growth could not be clearly observed.On the other hand,cell shapes considerably changed as ethanol volume fraction gradually increased.The cell integrity was broken down and plenty of cytoplasm was released at 8% ethanol addition.In addition,it was found that the cell utilization of glucose decreased with the ethanol volume fraction increased,After 32 h incubation,the residual glucose concentrations of control volume fraction and 3% ethanol addition reduced from 9.8 g·L-1 to 2.2 g·L-1 and 5.6 g·L-1,respectively,while no glucose consumption was remarkably detected at 6% ethanol addition.The membrane potential and membrane permeability of cell were further analyzed by Rh123 method and FDA method,respectively.The results showed that membrane potential reduced while membrane permeability enhanced with ethanol volume fraction increasing.At 3% and 6% ethanol addition,the membrane potential decreased 14.14% and 24.58% while the membrane permeability increased 6.58% and 18.37%,respectively.

关 键 词：简单节杆菌 乙醇体积分数 菌体生长 细胞性质 

分 类 号：Q939.129[生物学—微生物学]